Kingfisher duo instrument

Manufactured by Thermo Fisher Scientific

The KingFisher Duo is a compact, automated nucleic acid purification instrument from Thermo Fisher Scientific. It utilizes magnetic bead-based separation technology to extract and purify DNA or RNA samples from a variety of sample types. The KingFisher Duo offers a simple, reproducible, and high-throughput solution for nucleic acid purification in a wide range of applications.

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Lab products found in correlation

Superscript 2 reverse transcriptase, by Thermo Fisher Scientific (2 mentions) Rnase inhibitor, by Thermo Fisher Scientific (2 mentions) Oligo d t 18 primer, by New England Biolabs (2 mentions) Sera mag speedbead carboxylate modified magnetic particles hydrophobic, by GE Healthcare (2 mentions)

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KingFisher Duo (24 citations) KingFisher instrument (15 citations) KingFisher Duo Prime instrument (4 citations)

2 protocols using kingfisher duo instrument

Pro-B Cell RNA Isolation and qPCR

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Total RNA was prepared from ex vivo sorted pro‐B cells by using a semi‐automated RNA bead isolation method with Sera‐Mag SpeedBead Carboxylate‐Modified Magnetic Particles (Hydrophobic; GE Healthcare) run on the magnetic particle processor KingFisher Duo instrument (Thermo Fisher Scientific). The cDNA was synthesized using Oligo d(T)₁₈ primer (NEB) and SuperScript® II Reverse Transcriptase (Thermo Fisher Scientific) in the presence of RNase inhibitor (Thermo Fisher Scientific). Transcripts of the Gfp gene were amplified by qPCR using primers located in different exons (Dataset EV2) and normalized against the Tbp mRNA.

Hill L., Jaritz M., Tagoh H., Schindler K., Kostanova‐Poliakova D., Sun Q., Schwickert T.A., Leeb M, & Busslinger M. (2023). Enhancers of the PAIR4 regulatory module promote distal VH gene recombination at the Igh locus. The EMBO Journal, 42(15), e112741.

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Multiplex Transcriptome Analysis from B and T Cells

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Total RNA was prepared from distinct B and T cell types sorted from the bone marrow, thymus and spleen or from in vitro cultured v-Abl pro-B cell lines, using a semi-automated RNA bead isolation method with Sera-Mag SpeedBead Carboxylate-Modified Magnetic Particles (Hydrophobic; GE Healthcare) run on the magnetic particle processor KingFisher Duo instrument (Thermo Fisher Scientific). The cDNA was synthesized using Oligo d(T)₁₈ primer (NEB) and SuperScript II Reverse Transcriptase (Thermo Fisher Scientific) in the presence of RNase inhibitor (Thermo Fisher Scientific). The transcripts of selected genes were amplified by qPCR using primers located in different exons (Supplementary Table 4) and were normalized against the Tbp mRNA.

Hill L., Ebert A., Jaritz M., Wutz G., Nagasaka K., Tagoh H., Kostanova-Poliakova D., Schindler K., Sun Q., Bönelt P., Fischer M., Peters J.M, & Busslinger M. (2020). Wapl repression by Pax5 promotes V gene recombination by Igh loop extrusion. Nature, 584(7819), 142-147.

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