Anticoagulant rodenticides analysis was carried out following the method recently described by (Fourel et al., 2018 (link)). The ARs analysed in this study were three FGARs: warfarin, coumatetralyl, chlorophacinone and the five SGARs potentially used in European countries: bromadiolone, difenacoum, brodifacoum, flocoumafen, and difethialone. The chromatographic separation was achieved with a semi-porous Poroshell 120 StableBond C18 column (2.1*100 mm, 2.7 µm) and MS/MS detection was carried out by a 6410 B Triple Quadrupole from Agilent Technologies (Palo Alto, CA, United States) equipped with ElectroSpray Ionization source in negative mode. Two fragment ions were recorded in dynamic Multiple Reaction Monitoring mode and a calibration curve was built up for each analyte. The limits of quantification varied between 1–2 ng/g and the recovery rates were above 70%.
Poroshell 120 stablebond c18 column
Manufactured by Agilent Technologies
Sourced in United States
The Poroshell 120 StableBond C18 column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of compounds. It features a stable bonded stationary phase that provides reliable and consistent performance. The column is suitable for a variety of applications, including pharmaceutical, environmental, and food analysis.
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2 protocols using poroshell 120 stablebond c18 column
1
Quantifying Anticoagulant Rodenticides in Samples
2
HPLC-MS/MS Quantification Protocol
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Chromatographic analysis was performed on an Agilent 1200 series HPLC (Agilent Technologies, Santa Clara, CA, USA). Chromatographic separation was achieved on an Poroshell 120 StableBond C18 column (2.1*100mm, 2.7µm) from Agilent Technologies at ambient temperature with a mobile phase of A: 10mM ammonium acetate and B: acetonitrile. The elution gradient was: 20%B (0min), 30%B (0.1min), 40%B (0.5min), 50%B (5-11min), 90%B (11.5-12.5min), 20%B (13.5-25min) . The flow rate was 0.25mL/min. The injection volume was 1µL, and the autosampler tray was at ambient temperature.
MS/MS detection was carried out by a 6410B Triple Quadrupole from Agilent Technologies equipped with ElectroSpray Ionization source (ESI) in negative mode. The MS conditions were as follows: drying gas temperature 350°C, drying gas flow 8L/min, nebulizer pressure 40psi, and capillary voltage 4000V.
Fragment ions spectra were recorded in dynamic Multiple Reaction Monitoring (dMRM). Data collection and processing were performed with Masshunter TM Work-station from Agilent Technologies.
MS/MS detection was carried out by a 6410B Triple Quadrupole from Agilent Technologies equipped with ElectroSpray Ionization source (ESI) in negative mode. The MS conditions were as follows: drying gas temperature 350°C, drying gas flow 8L/min, nebulizer pressure 40psi, and capillary voltage 4000V.
Fragment ions spectra were recorded in dynamic Multiple Reaction Monitoring (dMRM). Data collection and processing were performed with Masshunter TM Work-station from Agilent Technologies.
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