A few hundred grams of joss fly ash were collected from a joss paper burning furnace located in a Chinese temple in a northern state of Malaysia. Sodium hydroxide, sulfuric acid, Tween-20, Tris-buffer, (3-aminopropyl)triethoxysilane (APTES), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC), and N-hydroxysuccinimide (NHS) were procured from Sigma Aldrich (USA). Enzyme-linked immunosorbent assay (ELISA) plates and horseradish peroxidase (HRP)-conjugated streptavidin were purchased from Thermo Scientific (USA). An ELASA coating buffer (5×) was procured from Biolegend (Japan). Bovine serum albumin (BSA) and 3,3′,5,5′-tetramethylbenzidine (TMB) were procured from Promega (USA). Biotinylated ampicillin aptamer with an ssDNA sequence of 5′-CACGGCATGGTGGGCGTCGTG-3′ was synthesized from a local supplier (Apical Scientific, Malaysia). The Escherichia coli and Bacillus subtilis strains were obtained from the School of Bioprocess Engineering, University Malaysia Perlis. A sterile disc was purchased from HiMedia Laboratories Pvt. Ltd. (Mumbai, India).
Enzyme linked immunosorbent assay elisa plates
Manufactured by Thermo Fisher Scientific
Sourced in United States
ELISA plates are a key component of the enzyme-linked immunosorbent assay (ELISA) technique. They are flat, solid-surface plates with multiple wells designed to hold small volumes of liquid samples. ELISA plates are used to detect and quantify specific proteins, antibodies, hormones, or other biomolecules in a given sample.
Automatically generated - may contain errors
Lab products found in correlation
3 3 5 5 tetramethylbenzidine (tmb), by Promega (2 mentions)
Bovine serum albumin (bsa), by Promega (2 mentions)
Sterile disc, by HiMedia (1 mentions)
1 ethyl 3 3 dimethylaminopropyl carbodiimide edc, by Merck Group (1 mentions)
Tris buffer, by Merck Group (1 mentions)
Horseradish peroxidase hrp conjugated streptavidin, by Thermo Fisher Scientific (1 mentions)
N hydroxysuccinimide nhs, by Merck Group (1 mentions)
Tween 20, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Sulfuric acid, by Merck Group (1 mentions)
3 aminopropyltriethoxysilane aptes, by Merck Group (1 mentions)
Ethanolamine, by Thermo Fisher Scientific (1 mentions)
Anti mouse igg horseradish peroxidase hrp, by Thermo Fisher Scientific (1 mentions)
K4fe cn 6, by Merck Group (1 mentions)
Diamond nanopowder, by Merck Group (1 mentions)
2 protocols using enzyme linked immunosorbent assay elisa plates
1
Joss Fly Ash Biodetection Assay
2
SARS-CoV-2 Spike Protein Immunosensor Development
Check if the same lab product or an alternative is used in the 5 most similar protocols
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A 10-µm gap-sized gold interdigitated electrode (AuIDE) with 125 electrode fingers connected by pads was purchased from Metrohm DropSens (Malaysia). His-tagged recombinant SARS-CoV-2 SP (native sequence: YP_009724390) produced in HEK293 cells was purchased from InvivoGen (China). Anti-SP antibody produced in mice was purchased from GeneTex, Inc. (North America). The recombinant SARS-CoV-2 nucleocapsid protein (NCP) and hemagglutinin (HA) of human influenza A for specificity determination of the immunosensor were procured from GeneTex, Inc. (North America) and Sino Biological Inc. (China), respectively. Diamond nanopowder was purchased from Sigma-Aldrich (USA). Phosphate-buffered saline (PBS, 1 M, pH 7.4), potassium hydroxide (KOH), 99% ethanol, 1,1′-carbonyldiimidazole (CDI), (3-glycidyloxypropyl)trimethoxysilane (GOPTS), potassium hexacyanoferrate (III), K3[Fe(CN)6] potassium hexacyanoferrate (II), and K4[Fe(CN)6] were procured from Sigma-Aldrich (USA). Ethanolamine (ETA) obtained from Fisher Scientific (UK) was used as a blocking agent. Enzyme-linked immunosorbent assay (ELISA) plates and anti-mouse IgG horseradish peroxidase (HRP) were procured from Thermo Scientific (USA). ELISA coating buffer (5 ×) was purchased from Biolegend (Japan). 3,3′,5,5′-tetramethylbenzidine (TMB) and bovine serum albumin (BSA) were purchased from Promega (USA).
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