cTnI: For rats, plasma samples were measured with Cardiac Injury Panel 3 (rat) Assay Kit and SECTOR® Imager 6000 (Meso Scale Discovery, MD, USA). For dogs and cynomolgus monkeys, serum samples were measured with Multiskan Ascent (Thermo Fischer Scientific, MA, USA).
cTnT: For rats, plasma samples were measured with Cardiac Injury Panel 3 (rat) Assay Kit and SECTOR® Imager 6000 (Meso Scale Discovery, MD, USA). For dogs and cynomolgus monkeys, blood samples were measured with Cobas h 232 (Roche Diagnostics GmbH, Mannheim, Germany).
CK and LDH: Serum samples were measured with JCA-BM6070 (Nihon Denshi, Tokyo, Japan) in both dogs and cynomolgus monkeys.