cTnI and cTnT levels were measured in rats, dogs and cynomolgus monkeys. CK and LDH levels were also measured in dogs and monkeys to monitor the effect by strenuous movement. The measurement methods are as follows.

cTnI: For rats, plasma samples were measured with Cardiac Injury Panel 3 (rat) Assay Kit and SECTOR® Imager 6000 (Meso Scale Discovery, MD, USA). For dogs and cynomolgus monkeys, serum samples were measured with Multiskan Ascent (Thermo Fischer Scientific, MA, USA).

cTnT: For rats, plasma samples were measured with Cardiac Injury Panel 3 (rat) Assay Kit and SECTOR® Imager 6000 (Meso Scale Discovery, MD, USA). For dogs and cynomolgus monkeys, blood samples were measured with Cobas h 232 (Roche Diagnostics GmbH, Mannheim, Germany).

CK and LDH: Serum samples were measured with JCA-BM6070 (Nihon Denshi, Tokyo, Japan) in both dogs and cynomolgus monkeys.

Note that all the testing methods were validated for their intra-assay precision, inter-assay precision, and frozen stability.
Nagata K., Sawada K., Minomo H., Sasaki D., Kazusa K, & Takamatsu K. (2017). Effects of repeated restraint and blood sampling with needle injection on blood cardiac troponins in rats, dogs, and cynomolgus monkeys. Comparative Clinical Pathology, 26(6), 1347-1354.
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