Ex vivo near infrared fluorescence imaging of rabbit aortas was performed on a Xenogen IVIS Spectrum Preclinical Imaging System (Perkin Elmer, Waltham, MA). Approximately 24 h before euthanasia, animals were injected with Cy5.5-labeled high-density lipoprotein (HDL), which was used to determine macrophage burden. Fluorescence images were obtained with the following excitation and emission band-pass filter parameters: λ excitation = 640 ± 18 nm, λ emission = 720 ± 10 nm. Images were subsequently analyzed by dividing the infrarenal abdominal aorta into 8 ROIs of the same size. Max radiant efficiency [(p/s/cm2/sr)/(μW/cm2)] for each ROI were recorded as a measure Cy5.5-HDL deposition in the aorta.
Xenogen ivis spectrum preclinical imaging system
Manufactured by PerkinElmer
The Xenogen IVIS Spectrum Preclinical Imaging System is a laboratory equipment designed for in vivo optical imaging. It enables the detection and quantification of bioluminescent and fluorescent signals from small animals or cell cultures.
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Lab products found in correlation
2 protocols using xenogen ivis spectrum preclinical imaging system
1
Ex vivo NIR Imaging of Rabbit Aortic Macrophages
2
Tissue Fluorescence Imaging Protocol
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