hDFs were cultured with 4.2 F MCMs on tissue culture-treated Thermanox Plastic Coverslips (Nunc) in DMEM with 10 v/v% FBS and 1000 U/mL P/S for 2 days. Cells were fixed on coverslips with 4% paraformaldehyde for 10 min at RT. Cells were further fixed in 0.7 M sodium cacodylate trihydrate (Sigma Aldrich) with 3 mM magnesium chloride (Sigma Aldrich) and 1.5 v/v% glutaraldehyde (Sigma-Aldrich) in DI water for 2 hrs at RT. Fixed cells were washed in 0.7 M sodium cacodylate with 2.5 w/v% sucrose 2X. Washed cells were dehydrated in 10 min subsequent incubations in 30, 50, 70, 95% ethanol in DI water. Dehydration was completed in 10 min subsequent incubations in 30, 50, 70, 95% hexamethyldisilazne (Sigma-Aldrich) in ethanol. Dehydrated cells were imaged on a LEO 1530 scanning electron microscope (Gemini) at 3kv. Cells and MCMs were pseudo colored in Adobe Photoshop to improve clarity of MCM and cellular regions.
Hexamethyldisilazne
Manufactured by Merck Group
Hexamethyldisilazane (HMDS) is a colorless, volatile liquid chemical compound. It is commonly used as a reagent in organic synthesis, particularly in the preparation of silyl ethers and as a protecting group for alcohols and phenols. HMDS can also be used as a surface-modifying agent in semiconductor fabrication processes.
Automatically generated - may contain errors
Lab products found in correlation
Thermanox plastic coverslip, by Thermo Fisher Scientific (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
Magnesium chloride, by Merck Group (1 mentions)
Sodium cacodylate trihydrate, by Merck Group (1 mentions)
Tetraethyl orthosilicate, by Merck Group (1 mentions)
Pluronic p123, by BASF (1 mentions)
Hexane, by Daejung Chemicals (1 mentions)
2 protocols using hexamethyldisilazne
1
Scanning Electron Microscopy of hDFs
2
Fabrication of Superhydrophobic Surfaces
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Pluronic
P123 ((poly(ethyleneoxide)-poly(propyleneoxide)-poly(ethyleneoxide))
was purchased from BASF (Korea). Tetraethyl orthosilicate (TEOS, >99%)
and hexamethyl disilazne (99.9%) were purchased from Sigma-Aldrich
(Korea). Octadecyl-triethoxysilane (OD-TES), hexamethyldisilazne (HMDS),
(3,3,3-trifluoropropyl) trimethoxy silane (TFP-TMOS), and tridecafluoro-1,1,2,2-tetrahydrooctyltrimethoxysilane
(TDF-TMOS) were purchased from Gelest (USA). Hexane (95%) was purchased
from Daejung Co., Ltd. (Korea).
P123 ((poly(ethyleneoxide)-poly(propyleneoxide)-poly(ethyleneoxide))
was purchased from BASF (Korea). Tetraethyl orthosilicate (TEOS, >99%)
and hexamethyl disilazne (99.9%) were purchased from Sigma-Aldrich
(Korea). Octadecyl-triethoxysilane (OD-TES), hexamethyldisilazne (HMDS),
(3,3,3-trifluoropropyl) trimethoxy silane (TFP-TMOS), and tridecafluoro-1,1,2,2-tetrahydrooctyltrimethoxysilane
(TDF-TMOS) were purchased from Gelest (USA). Hexane (95%) was purchased
from Daejung Co., Ltd. (Korea).
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