Igf2bp2 sirna

Manufactured by GenePharma

Sourced in United States, China

IGF2BP2-siRNA is a laboratory tool used to study the function of the IGF2BP2 gene. It is a synthetic small interfering RNA (siRNA) molecule that targets the IGF2BP2 mRNA, leading to its degradation and reduced expression of the IGF2BP2 protein. This product is intended for research use only.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Mettl3 sirna, by GenePharma (1 mentions) Pcdna3.1 plasmid, by Addgene (1 mentions) Lipo2000, by Addgene (1 mentions)

Close spelling variants of this product

IGF2BP2 SiRNAs

2 protocols using igf2bp2 sirna

Silencing IGF2BP2 in Colorectal Cancer

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Lipofectamine 3000 (Invitrogen, USA), was used for IGF2BP2-siRNA (GenePharma, Shanghai, China) transfection. After 48 or 72 h of transfection, cells were analyzed by qRT-PCR or western blotting to confirm the transfection efficiency. Then, the IGF2BP2-shRNA lentivirus was inserted into the pds401_pL-U6-shRNA-Luc- ccdB-puro vector (TSINKGE, Beijing, China), and the infected CRC cells were grown in a culture medium containing 5 µg/ml polybrene. IGF2BP2-knockdown CRC cells were rescued and virus-infected cells were transfected using a TFRC- overexpressing plasmid. The stably transfected cell lines were selected using 10 µg/ml puromycin. All the sequences mentioned above are presented in Table S1.

Liu T.Y., Hu C.C., Han C.Y., Mao S.Y., Zhang W.X., Xu Y.M., Sun Y.J., Jiang D.B., Zhang X.Y., Zhang J.X., Wang J., Qiao X.P., Pan J.Y., Yang S.Y, & Yang K. (2023). IGF2BP2 promotes colorectal cancer progression by upregulating the expression of TFRC and enhancing iron metabolism. Biology Direct, 18, 19.

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Investigating Gene Expression Modulation

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TRIM37 siRNA (siR-1, 5'-GGAGAAGAUUCAGAAUGAATT-3'; siR-2, 5'-CCAGUAGUUUACUAGACAUTT-3'; siR-3, 5'-GCCUUGAUACAUGGCAGUATT-3'), METTL3 siRNA (siR#1, 5'-GCUGCACUUCAGACGAAUUTT-3'; siR#2, 5'-GGAUACCUGCAAGUAUGUUTT-3'), and IGF2BP2 siRNA (siR&1, 5'-CCCAGUUUGUUGGUGCCAUTT-3'; siR&2, 5'-GCGAAAGGAUGGUCAUCAUTT-3') were synthesized by Genepharm (Shanghai, China). For ectopic expression of TRIM37, GPX4, METTL3, or IGF2BP2, the coding sequences were cloned into pcDNA3.1 plasmids (Addgene, USA).
Transfections were performed with Lipo2000.

Pan C., xiangbin K., Wu Z., & Fei Q. (2022). The role of hepatitis B virus surface protein in inducing Sertoli cell ferroptosis.

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