For whole mouse imaging, a custom-built setup was used. Lumics laser units: LU1064DLD350-S70AN03 (35 W) “1064 nm”; LU0980D350-D30AN (35W) “980 nm”, and LU0785DLU250-S70AN03 (25 W) “785 nm” were used for excitation. Laser modules are specced to ± 10 nm. Laser outputs were coupled in a 4 × 1 fan-out fiber-optic bundle (Thorlabs BF46LS01) of 600 μm core diameter for each optical path. The output from the fiber was fixed in an excitation cube (Thorlabs KCB1EC/M), reflected off of a mirror (Thorlabs BBE1-E03), and passed through a positive achromat (Thorlabs AC254–050-B), SP filter (if necessary) and a ground glass diffuser (Thorlabs DG10–120-MD) or an engineered diffuser (Thorlabs ED1-S20-MD) to provide uniform illumination over the working area. In a typical experiment, the excitation flux at the object was adjusted to be close to 100 mWcm−2 with an error of ± 3% (power density used is defined separately in each experiment). The working area was covered by a heating mat coated with blackout fabric (Thorlabs BK5). Emitted light was directed onto an Allied Vision Goldeye G-032 Cool TEC2 camera with a sensor temperature set point of −20 °C.
Dg10 120 md
Manufactured by Thorlabs
The DG10-120-MD is a mechanical diaphragm vacuum pump that can achieve a maximum vacuum level of 120 mbar absolute. It is designed for general-purpose vacuum applications in laboratory settings.
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2 protocols using dg10 120 md
1
Whole Mouse Imaging with Multiwavelength Excitation
2
Whole Mouse Imaging with Multiwavelength Excitation
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For whole mouse imaging, a custom-built setup was used. Lumics laser units: LU1064DLD350-S70AN03 (35 W) “1064 nm”; LU0980D350-D30AN (35W) “980 nm”, and LU0785DLU250-S70AN03 (25 W) “785 nm” were used for excitation. Laser modules are specced to ± 10 nm. Laser outputs were coupled in a 4 × 1 fan-out fiber-optic bundle (Thorlabs BF46LS01) of 600 μm core diameter for each optical path. The output from the fiber was fixed in an excitation cube (Thorlabs KCB1EC/M), reflected off of a mirror (Thorlabs BBE1-E03), and passed through a positive achromat (Thorlabs AC254–050-B), SP filter (if necessary) and a ground glass diffuser (Thorlabs DG10–120-MD) or an engineered diffuser (Thorlabs ED1-S20-MD) to provide uniform illumination over the working area. In a typical experiment, the excitation flux at the object was adjusted to be close to 100 mWcm−2 with an error of ± 3% (power density used is defined separately in each experiment). The working area was covered by a heating mat coated with blackout fabric (Thorlabs BK5). Emitted light was directed onto an Allied Vision Goldeye G-032 Cool TEC2 camera with a sensor temperature set point of −20 °C.
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