Tsa plus tmr

Manufactured by PerkinElmer

The TSA Plus TMR is a laboratory instrument used for the detection and quantification of analytes in various sample types. It utilizes a time-resolved fluorescence (TRF) measurement technique to provide accurate and sensitive results. The core function of the TSA Plus TMR is to perform immunoassays and other types of analysis that require this measurement approach.

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Lab products found in correlation

Peroxidase affinipure f ab 2 donkey anti rat igg antibody h l, by Jackson ImmunoResearch (2 mentions) Maid 2, by Thermo Fisher Scientific (2 mentions) Oct compound, by Sakura Finetek (2 mentions) Paraformaldehyde (pfa), by Fujifilm (2 mentions) Cy3 conjugated goat anti rabbit igg antibody, by Merck Group (2 mentions) Ab93862, by Abcam (2 mentions)

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TSA Plus TMR System (2 citations)

2 protocols using tsa plus tmr

Immunofluorescent Staining of AID and GDF-9 in Mouse Tissues

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The small intestines and ovaries of mice were fixed for 2 h at 4 °C in a fresh solution of 4% paraformaldehyde (Wako, Japan). The samples were then washed in PBS, incubated overnight at 4 °C in a solution of 30% sucrose, and embedded in OCT compound (Sakura Finetek). The tissue segments were sectioned with a cryostat at 8 μm and stained for AID using a tyramide signal amplification system kit with TSA Plus TMR (PerkinElmer)^{42 (link)}. GDF-9 were assessed by indirect immunofluorescent staining. Immunohistochemical staining was performed using a standard avidin–biotin complex peroxidase method, as described previously^{43 (link)}. The primary antibodies used in this study were as follows: rat monoclonal anti-AID antibody MAID-2 (eBioscience, 14-5959-80)^{44 (link)}, rat monoclonal anti-AID antibody (Merck Millipore MABF63, clone 328.8), rabbit polyclonal anti-GDF9 antibody (abcam, ab93862), and rat IgG2b kappa isotype control (eBioscience, 16-4031-81, clone eB149/10H5). The secondary antibodies used in this study were as follows: Peroxidase AffiniPure F(ab’)₂ donkey anti-rat IgG antibody (H + L) (Jackson Immunoresearch, 712-036-153), Cy3-conjugated goat anti-rabbit IgG antibody (Chemicon, AP132C).

Iizuka T., Wakae K., Ono M., Suzuki T., Mizumoto Y., Kitamura K., Horike S.I., Muramatsu M, & Fujiwara H. (2021). Activation-induced cytidine deaminase is a possible regulator of cross-talk between oocytes and granulosa cells through GDF-9 and SCF feedback system. Scientific Reports, 11, 3833.

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Immunohistochemical Analysis of AID and GDF-9 in Mice

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The small intestines and ovaries of mice were fixed for 2 hours at 4°C in a fresh solution of 4% paraformaldehyde (Wako, Japan). The samples were then washed in PBS, incubated overnight at 4°C in a solution of 30% sucrose, and embedded in OCT compound (Sakura Finetek). The tissue segments were sectioned with a cryostat at 8 μm and stained for AID using a tyramide signal amplification system kit with TSA Plus TMR (PerkinElmer) 42 (link) . GDF-9 were assessed by indirect immunofluorescent staining. Immunohistochemical staining was performed using a standard avidin-biotin complex peroxidase method, as described previously 43 (link) . The primary antibodies used in this study were as follows: rat monoclonal anti-AID antibody MAID-2 (eBioscience, 14-5959-80) 44 (link) , rat monoclonal anti-AID antibody (Merck Millipore MABF63, clone 328.8), rabbit polyclonal anti-GDF9 antibody (abcam, ab93862), and rat IgG2b kappa isotype control (eBioscience, 16-4031-81, clone eB149/10H5).
The secondary antibodies used in this study were as follows: Peroxidase AffiniPure F(ab')2 donkey anti-rat IgG antibody (H+L) (Jackson Immunoresearch, 712-036-153), Cy3conjugated goat anti-rabbit IgG antibody (Chemicon, AP132C).

Iizuka T., Wakae K., Suzuki T., Ono M., Kitamura K., Horike S., Muramatsu M., & Fujiwara H. (2020). Activation-Induced Cytidine Deaminase is A Possible Regulator of Cross-Talk Between Oocytes and Granulosa Cells Through GDF-9 and SCF Feedback System.

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