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Plenti pgk v5 luc puro w543 1 vector

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The PLenti PGK V5-LUC-Puro (W543–1) vector is a lentiviral expression vector that allows for the expression of a luciferase reporter gene and a puromycin resistance gene under the control of the PGK promoter. The vector can be used for the stable transduction and selection of mammalian cell lines.

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Lab products found in correlation

Phiv h2bmrfp vector, by Addgene (2 mentions)

Spelling variants of this product

PLenti-puro (24 citations) PLenti-puro vector (13 citations)

2 protocols using plenti pgk v5 luc puro w543 1 vector

1

Stable Ectopic Expression of PKR Variants

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Stable overexpression and re-expression of PKR (wild type, 3A:S83A/S456A/S542A mutant, kinase-dead: K296R mutant) in PKR-KO cells were achieved by lentivirus-mediated infection and selection. Ectopic expression of Bcl2 was also achieved by a lentivirus-mediated approach. The transduced cells were then selected with 800 μg/ml of neomycin (at 48 hours post-infection) to establish cell lines stably expressing exogenous proteins. HeLa-RFP and HeLa-PKR-KO-RFP cell lines were achieved by lentivirus-mediated infection of pHIV-H2BmRFP vector (Addgene 18982). The transduced and RFP-positive cells were selected by flow cytometry-based sorting. SKOV3-PKR-KO cells expressing luciferase were obtained by infection of pLenti PGK V5-LUC-Puro (W543–1) vector (Addgene 19360) and followed by puromycin selection.
Yin L., Zeng Y., Zeng R., Chen Y., Wang T.L., Rodabaugh K.J., Yu F., Natarajan A., Karpf A.R, & Dong J. (2021). Protein Kinase RNA-activated Controls Mitotic Progression and Determines Paclitaxel Chemosensitivity through B-cell lymphoma 2 in Ovarian Cancer. Oncogene, 40(50), 6772-6785.
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2

Stable Ectopic Expression of PKR Variants

Check if the same lab product or an alternative is used in the 5 most similar protocols
Stable overexpression and re-expression of PKR (wild type, 3A:S83A/S456A/S542A mutant, kinase-dead: K296R mutant) in PKR-KO cells were achieved by lentivirus-mediated infection and selection. Ectopic expression of Bcl2 was also achieved by a lentivirus-mediated approach. The transduced cells were then selected with 800 μg/ml of neomycin (at 48 hours post-infection) to establish cell lines stably expressing exogenous proteins. HeLa-RFP and HeLa-PKR-KO-RFP cell lines were achieved by lentivirus-mediated infection of pHIV-H2BmRFP vector (Addgene 18982). The transduced and RFP-positive cells were selected by flow cytometry-based sorting. SKOV3-PKR-KO cells expressing luciferase were obtained by infection of pLenti PGK V5-LUC-Puro (W543–1) vector (Addgene 19360) and followed by puromycin selection.
Yin L., Zeng Y., Zeng R., Chen Y., Wang T.L., Rodabaugh K.J., Yu F., Natarajan A., Karpf A.R, & Dong J. (2021). Protein Kinase RNA-activated Controls Mitotic Progression and Determines Paclitaxel Chemosensitivity through B-cell lymphoma 2 in Ovarian Cancer. Oncogene, 40(50), 6772-6785.
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