Renalase and NF-κB levels were analyzed using western blot similar to that described previously (Wang et al., 2014a (link)). The primary antibodies, anti-renalase, anti-caspase3, anti-Phospho-IκBα (Ser32) and anti-GAPDH were from Abcam (Cambridge, MA, USA) (rabbit anti-renalase monoclonal antibody, 1:500 dilution), Cell Signaling Technology (Danvers, MA, USA) (rabbit anti-Phospho-IκBα (Ser32) monoclonal antibody, 1:500 dilution; rabbit anti-caspase3 polyclonal antibodies, 1:1000 dilution) and Santa Cruz Biotechnology (Santa Cruz, CA, USA) (goat anti-GAPDH polyclonal antibody, 1:5000 dilution), respectively. The secondary antibodies were from Santa Cruz (horseradish peroxidase–conjugated goat anti-mouse and anti-rabbit IgG).
Anti renalase
Manufactured by Abcam
Sourced in United States
Anti-renalase is a primary antibody that targets the renalase protein. Renalase is an enzyme involved in the metabolism of catecholamines. The function of this antibody is to detect and bind to the renalase protein for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Anti gapdh, by Abcam (2 mentions)
Anti caspase 3, by Abcam (1 mentions)
Horseradish peroxidase conjugated goat anti mouse and anti rabbit igg, by Santa Cruz Biotechnology (1 mentions)
Sc 48166, by Santa Cruz Biotechnology (1 mentions)
A5441, by Merck Group (1 mentions)
Anti β actin, by Abcam (1 mentions)
Chemidoc xrs system, by Bio-Rad (1 mentions)
Anti hif 1α, by Abcam (1 mentions)
2 protocols using anti renalase
1
Renalase and NF-κB Expression Analysis
2
Quantifying Renalase and HIF-1α Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The relative protein levels of renalase and HIF-1α were analysed using Western blot analysis similar to what was described previously 17 (link),18 (link). The primary antibodies, anti-renalase, anti-HIF-1α, anti-β actin and anti-GAPDH were from Abcam (goat anti-renalase polyclonal antibody, ab31291, 1:500 dilution, for HK2 Western), (rabbit anti-renalase monoclonal antibody, 1:500 dilution, for rat tissues Western), Novus Biologicals (Littleton, CO, USA) (NB100-105, mouse anti-HIF-1α monoclonal antibody, 1:500 dilution), Sigma-Aldrich (A5441, mouse anti-β-actin monoclonal antibody, 1:10,000 dilution) and Santa Cruz (sc-48166, goat anti-GAPDH polyclonal antibody, 1:5000 dilution) respectively. The secondary antibodies were from Santa Cruz (horseradish peroxidase-conjugated anti-rabbit and anti-goat IgG) or Sigma-Aldrich (horseradish peroxidase-conjugated antimouse IgG). GAPDH and β-actin were used as internal control for renalase and HIF-1α respectively. All the data were obtained from ChemiDoc XRS+ System (Bio-Rad, Hercules, CA, USA) and band intensity was analysed using Image Lab 4.0.1 software.
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