Cell proliferation was analyzed using a commercial CCK-8 assay kit (#C0038, Beyotime) and EdU detection kit (#C0075S, Beyotime). Fluorescence-activated cell sorting (FACS) was used to assess the cell cycle with a PI staining kit (#R40432, Sigma). Cell invasion was assessed by the transwell assay with a 6-well insert device (8 μm pore size; Corning Life Sciences, Bedford, MA) and Biocoat Matrigel (BD Biosciences) according to the manufacturer’s instructions.
6 well insert device
Manufactured by Corning
Sourced in United Kingdom, United States
The 6-well insert device is a laboratory equipment designed for cell culture applications. It consists of a multiwell plate with six individual wells, each containing a porous membrane insert. These inserts allow for the co-culture of different cell types or the separation of cells from the culture medium, facilitating various experimental setups. The core function of this device is to provide a controlled and versatile in vitro environment for cell-based studies and assays.
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2 protocols using 6 well insert device
1
Cell Proliferation, Cycle, and Invasion Assays
2
Comprehensive Cell Characterization Assays
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Cell proliferation was analyzed using a commercial CCK-8 assay kit (#C0038, Beyotime). Fluorescence-activated cell sorting (FACS) was used to assess apoptosis with the Annexin V-FITC/PI staining kit (Mbchem). Cell invasion was assessed by the Transwell assay with a 6-well insert device (8 μm pore size; Corning Life Sciences, Bedford, MA, USA) and Biocoat Matrigel (BD Biosciences) according to the manufacturer’s instructions.
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