Goat anti rabbit antibodies

Manufactured by Vector Laboratories

Sourced in United States

Goat anti-rabbit antibodies are secondary antibodies produced in goats that specifically recognize and bind to rabbit primary antibodies. These antibodies can be used to detect and amplify signals from rabbit-derived primary antibodies in various immunoassays and immunochemical techniques.

Automatically generated - may contain errors

Lab products found in correlation

Streptavidin peroxidase complex, by Vector Laboratories (1 mentions) Biotinylated horse anti mouse immunoglobulin g, by Vector Laboratories (1 mentions) Rabbit anti erk1 2, by Cell Signaling Technology (1 mentions) Protease and phosphatase inhibitor cocktail, by Merck Group (1 mentions) Rabbit anti gapdh, by Cell Signaling Technology (1 mentions) Rabbit anti fibronectin, by Abcam (1 mentions) Mouse anti sma, by Merck Group (1 mentions) Rabbit anti smad2 3, by Cell Signaling Technology (1 mentions) Horse anti mouse, by Vector Laboratories (1 mentions) Rabbit anti smad2, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Biotinylated anti-rabbit or anti-goat antibodies (2 citations) Goat anti-rabbit antibodies coupled to horseradish peroxidase (2 citations) Biotinylated goat anti-mouse (BA-9200) and goat anti-rabbit (BA-1000) secondary antibodies (2 citations) Biotin-labeled rabbit anti-goat antibodies (2 citations)

2 protocols using goat anti rabbit antibodies

Immunohistochemical Staining of Astrocytes and Microglia

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunohistochemistry was performed according to our previously published procedure (24 (link)). Briefly, immunostaining was performed using mouse anti-glial fibrillary acidic protein (GFAP; 1:800; cat no. MAB360; EMD Millipore, Billerica, MA, USA) for astrocytes and rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1; 1:800; cat no. 019-19741; Wako Pure Chemical Industries, Ltd., Osaka, Japan) for microglia overnight at 4°C. Subsequently, samples were incubated with biotinylated horse anti-mouse immunoglobulin G (1:250; cat no. BA2000; Vector Laboratories, Inc., Burlingame, CA, USA) or goat anti-rabbit antibodies (1:250; cat no. BA1000; Vector Laboratories, Inc.) for 2 h at room temperature, and streptavidin peroxidase complex (1:200; Vector Laboratories, Inc.) for 1 h at room temperature. To establish the specificity of the immunostaining, a negative control test was performed and resulted in the absence of immunoreactivity in all structures.

Ahn J.H., Shin M.C., Park J.H., Kim I.H., Cho J.H., Lee T.K., Lee J.C., Chen B.H., Shin B.N., Tae H.J., Park J., Choi S.Y., Lee Y.L., Kim D.W., Kim Y.H., Won M.H, & Cho J.H. (2017). Effects of long-term post-ischemic treadmill exercise on gliosis in the aged gerbil hippocampus induced by transient cerebral ischemia. Molecular Medicine Reports, 15(6), 3623-3630.

+ Open protocol

+ Expand

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were lysed in RIPA buffer complemented with phosphatase and protease inhibitor cocktails (Sigma) for protein extraction. Protein concentrations were determined by Bradford assays, and ~ 30 μg per sample was used for Western blotting. For non-phosphorylated protein detection, membranes were blocked and antibodies were added in 5 % milk tris-buffered saline with 0.1 % Tween 20 (TBST), and for phosphorylated protein detection, 5 % bovine serum albumin (BSA) TBST was used. Membranes were incubated with primary antibodies at 4 °C overnight and then with secondary antibody at room temperature for one hour. Primary antibodies: 1:1000 mouse anti-SMA (Sigma, A5228), 1:1000 rabbit anti-phospho-Ser 465/467 SMAD2 (pSMAD2) (Cell Signaling, 3108), 1:1000 rabbit anti-phospho-Ser 423/425 SMAD3 (pSMAD3) (Millipore, 0713-89), 1:1000 rabbit anti-SMAD2 (Cell Signaling, 5339), 1:1000 rabbit anti-SMAD2/3 (Cell Signaling, 8685), 1:1000 rabbit anti-fibronectin (Abcam, ab2413), 1:1000 rabbit anti-bFGF (Sigma), 1:1000 rabbit anti-phospho-Thr 202/204 ERK (pERK) 1/2 (Cell Signaling, 4370), 1:2000 rabbit anti-ERK1/2 (Cell Signaling, 9102), 1:1000 rabbit anti-VEGF receptor 2 (VEGFR2) (Cell Signaling, 55B11), and 1:2500 rabbit anti-GAPDH (Cell Signaling, 5174). Secondary HRP-conjugated antibodies: 1:10,000 horse anti-mouse and 1:10,000 goat anti-rabbit antibodies (Vector Laboratories).

Xiao L., Kim D.J., Davis C.L., McCann J.V., Dunleavey J.M., Vanderlinden A., Xu N., Pattenden S.G., Frye S.V., Xu X., Onaitis M., Monaghan-Benson E., Burridge K, & Dudley A.C. (2015). Tumor endothelial cells with distinct patterns of TGFβ-driven endothelial-to-mesenchymal transition. Cancer research, 75(7), 1244-1254.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!