Spheroids were treated upon plating with a panel of antagonists or agonists at the following final concentrations: 20 nM batimastat (R&D 2961/10), 40 μM ciliobrevin D (Sigma 250,401), 10 μM defactinib (Med Chem HY-122899), 30 nM pemigatinib (Med Chem HY-109099), 1 μM volsertib (Selleck S2235), 4 mM 2-Deoxy-Glucose (Sigma D8375), 10 μM GC7 (EMD Millipore 259,545), 1 μg/mL follistatin (R&D 769-FS-025), 1 μg/mL collagen XV (9854-CL-050), 5 μg/mL collagen I (Sigma C2249), and 10 ng/mL activin-A (R&D 338-AC-010). For integrin inhibition, PDAC-MSC multicellular spheroids were treated with 2 mM EGTA or the mAb13 anti-ITGB1 antibody (Sigma MABT821) at a 1:10 dilution.
Volsertib
Manufactured by Selleck Chemicals
Volsertib is a lab equipment product that serves as a protein kinase inhibitor. It functions by inhibiting the activity of various protein kinases, which are enzymes involved in cellular signaling pathways. The core function of Volsertib is to modulate the activity of these protein kinases for research and experimental purposes.
Automatically generated - may contain errors
Lab products found in correlation
Collagen 1, by Merck Group (1 mentions)
Ciliobrevin d, by Merck Group (1 mentions)
2 deoxyglucose, by Merck Group (1 mentions)
Follistatin, by R&D Systems (1 mentions)
Activin a, by R&D Systems (1 mentions)
Batimastat, by R&D Systems (1 mentions)
Colchicine, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Dinaciclib, by Selleck Chemicals (1 mentions)
Bi2536, by Selleck Chemicals (1 mentions)
2 protocols using volsertib
1
Spheroid Antagonist and Agonist Treatment
2
High-Throughput Screening of Chemical Libraries
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The screening libraries comprised 10 371 compounds from the Rockefeller University High-Throughput Screening Resource Center (http://www.rockefeller.edu/htsrc/libraries ). They were resuspended in DMSO (Sigma-Aldrich), and stock solutions were stored at −20°C.
NOC and colchicine were purchased from Sigma-Aldrich, paclitaxel was purchased from Cytoskelton Inc., BI2536, Volsertib, Barasartib and Dinaciclib were purchased from Selleck Chemicals. All chemicals were dissolved in DMSO, kept at −20°C, and used in a 0.25–1% DMSO solution.
NOC and colchicine were purchased from Sigma-Aldrich, paclitaxel was purchased from Cytoskelton Inc., BI2536, Volsertib, Barasartib and Dinaciclib were purchased from Selleck Chemicals. All chemicals were dissolved in DMSO, kept at −20°C, and used in a 0.25–1% DMSO solution.
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