Anti osmr

Manufactured by Abcam

Sourced in United Kingdom

Anti-OSMR is a primary antibody that specifically recognizes the Oncostatin M receptor (OSMR). OSMR is a type I cytokine receptor that binds Oncostatin M, a member of the IL-6 family of cytokines. This antibody can be used in various applications to detect and study the OSMR protein.

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2 protocols using anti osmr

Western Blot Analysis Protocol

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Western blot analysis was carried out as described previously^{16 (link)}. Antibodies used in western blot are listed as follows: anti-OSMR (Abcam, ab210771, 1 : 1000) (Cambridge, United Kingdom), anti-HOXC10 (Abcam, ab153904, 1 : 1000), anti-SCARA3 (Abcam, ab96205, 1 : 000), anti-SLC39A10 (Abcam, ab83947, 1 : 1000), anti-β-Actin (Cell Signaling Technology, #8457, 1 : 1000) (Danvers, Massachusetts), and horseradish peroxidase-conjugated anti-rabbit IgG (Cell Signaling Technology, #7074, 1 : 5000). The relative expression of target proteins was quantified in comparison with β-Actin using Image Lab software (Bio-Rad Laboratories, Berkeley, California) and was then normalized to the corresponding expression in HEB cells.

Cao M., Cai J., Yuan Y., Shi Y., Wu H., Liu Q., Yao Y., Chen L., Dang W., Zhang X., Xiao J., Yang K., He Z., Yao X., Cui Y., Zhang X, & Bian X. (2019). A four-gene signature-derived risk score for glioblastoma: prospects for prognostic and response predictive analyses. Cancer Biology & Medicine, 16(3), 595-605.

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Immunoblot Antibody Dilutions for Protein Analysis

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Immunoblot studies were performed using the following antibodies at 1:1000 dilution: anti-DAP5 (BD Biosciences 610742); anti-eIF3d (Bethyl A301-758A); anti-eIF4A1 (Cell Signaling 2490); anti-eIF4G1 (Cell Signaling 2858); anti-rpS19 (Bethyl A304-002A); anti-eIF4E (Bethyl A301-154A); anti-HA epitope tag (Abcam 18181); anti-c-Jun (Cell Signaling 9165); anti-CDK12 (Cell Signaling 11973); anti-MMP1 (Abcam 137332); anti-GAPDH (Cell Signaling 2118S); anti-4E-BP1 (Cell Signaling 9644); anti-Lumin A (Abcam 226189); anti-AUF1 (EMD Millipore 07260); anti FXR1 (Cell Signaling 12295); anti FXR2 (Cell Signaling 7098); and anti-PABP (Cell Signaling 4992). The following antibodies were used at 1:500 dilution: anti-ETS1 (Abcam 26096); anti-OSMR (Abcam 210771); anti-EGFR (Cell Signaling 4257); anti-SERPINE2 (Abcam 75348); anti-ITGV (Abcam 124968); and anti-p53-BP1 (Abcam 21083).

de la Parra C., Ernlund A., Alard A., Ruggles K., Ueberheide B, & Schneider R.J. (2018). A widespread alternate form of cap-dependent mRNA translation initiation. Nature Communications, 9, 3068.

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