Uv 510 confocal microscope

Manufactured by Zeiss

The Zeiss UV 510 confocal microscope is a laboratory instrument designed for high-resolution imaging of biological samples. It utilizes ultraviolet (UV) excitation wavelengths to enable visualization and analysis of cellular structures and components. The core function of the UV 510 is to provide researchers with a versatile platform for advanced fluorescence microscopy studies.

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Lab products found in correlation

Accuri c6 flow cytometer, by BD (3 mentions) Lsm 710 confocal microscope, by Zeiss (3 mentions) Plan neofluar, by Zeiss (1 mentions) Fitc anti mouse cd3, by BioLegend (1 mentions) Plan apochromat 63 1.4 oil lens, by Zeiss (1 mentions) Glass bottom dishes, by Ibidi (1 mentions) Plan neofluar 40 1.3 oil lens, by Zeiss (1 mentions) Plan apochromat 63 1.40 oil lens, by Zeiss (1 mentions)

Close spelling variants of this product

Zeiss UV Meta 510 confocal microscope LSM 510 UV Meta confocal microscope LSM 510 UV confocal microscope Zeiss 510 confocal microscope Confocal microscope

5 protocols using uv 510 confocal microscope

Visualization and Quantification of Gene Silencing

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For silencing experiments, cells were visualized using a UV 510 confocal microscope (Carl Zeiss, Oberkochen) and a Plan-Neofluar 40×/1.3 oil lens. To image GFP fluorescence, a 488 nm laser beam was used for excitation, and detection was acquired using a BP filter 505–550 nm. Flow cytometry was performed on a BD Accuri C6 flow cytometer; CellQuest or the CFlow Sampler software was used to obtain the gMFI. To determine statistical significance, treatments were compared to cells-only using a one-way analysis of variance (ANOVA), followed by a Dunnett’s multiple comparisons test performed in GraphPad Prism version 9.0.0 for Windows, GraphPad Software, San Diego, California, USA, www.graphpad.com. Data are expressed as the mean ± SEM for n = 20,000 events per treatment. A P-value of <0.05 was considered statistically significant.

Chandler M., Minevich B., Roark B., Viard M., Johnson M.B., Rizvi M.H., Deaton T.A., Kozlov S., Panigaj M., Tracy J.B., Yingling Y.G., Gang O, & Afonin K.A. (2021). Controlled Organization of Inorganic Materials Using Biological Molecules for Activating Therapeutic Functionalities. ACS applied materials & interfaces, 13(33), 39030-39041.

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Multicolor Neutrophil Imaging and Analysis

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For neutrophil mitochondrial images, isolated neutrophils were incubated in complete sea horse media (2 mM glutamine and 25 mM glucose) for 20 min at 37 °C with mitotracker CMX ROS (100 nM). Cells were washed in PBS and incubated with Hoechst (2 μg/ml) for 20 min with 5 °C after which cells were washed in PBS. Neutrophils were placed in a cytospin cartridge and spun at 800 rpm and spun for 3 min. Cells were then fixed in 4% PFA for 20 min, washed and a coverslip was added. A Zeiss UV-510 confocal microscope and 63× oil immersion objective lens was used to capture images using differential interference contrast, 405 and 561 laser paths (pinhole size = 1 airy unit).
Tumour sections were processed fresh-frozen sections and were washed with PBS, mild-fixed with acetone at 4 C, permeablised with 0.05% Triton in PBS and blocked with 5% BSA in PBS. Sections incubated at room temperature for 1 h with the following antibodies (1:100, all from Biolegend): FITC anti-mouse CD3, PerCP/Cy5.5 anti-mouse GR-1, APC anti-mouse c-Kit and counterstained with DAPI. Images were analysed using Fiji (ImageJ).

Rice C.M., Davies L.C., Subleski J.J., Maio N., Gonzalez-Cotto M., Andrews C., Patel N.L., Palmieri E.M., Weiss J.M., Lee J.M., Annunziata C.M., Rouault T.A., Durum S.K, & McVicar D.W. (2018). Tumour-elicited neutrophils engage mitochondrial metabolism to circumvent nutrient limitations and maintain immune suppression. Nature Communications, 9, 5099.

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Silencing Experiments Visualized by Confocal Microscopy

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The silencing experiments were visualized using a UV 510 confocal microscope (Carl Zeiss, Oberkochen) and a Plan-Neofluar 40×/1.3 Oil lens. Flow Cytometry. BD Accuri C6 flow cytometer was used for all experiments. CellQuest or the CFlow Sampler software was used to retrieve the geometric mean fluorescence intensity (gMFI) and the standard error of the mean.

Bui M.N., Johnson M.B., Viard M., Satterwhite E., Martins A.N., Li Z., Marriott I., Afonin K.A, & Khisamutdinov E.F. (2017). Versatile RNA tetra-U helix linking motif as a toolkit for nucleic acid nanotechnology. Nanomedicine : nanotechnology, biology, and medicine, 13(3), 1137-1146.

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Quantifying Fluorescent Nanoparticles by Microscopy and Flow Cytometry

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Experiments were performed using a UV 510 confocal microscope (Carl Zeiss, Oberkochen) and a Plan-Apochromat 63×/1.4 oil lens. To image QD545, a 488 nm laser beam was used for excitation and a BP filter 505–550 nm for detection. To image QD605, a 561 nm laser beam was used for excitation and a BP filter 575–615 nm for detection. All images were taken with a pinhole adjusted to 1 Airy unit. Flow cytometry was performed on a BD Accuri C6 flow cytometer; CellQuest or the CFlow Sampler software was used to obtain the gMFI. Data are expressed as the mean ± SEM for n = 20,000 events per treatment.

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Visualizing Silencing and Uptake of Nanoconstructs

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The silencing experiments were visualized using a UV 510 confocal microscope (Carl Zeiss, Oberkochen) and a Plan-Neofluar 40×/1.3 oil lens. MDA-MB-231 eGFP cells were plated in glass bottom dishes (Ibidi, Madison), transfected as described above. 3 days upon transfection, the cells were washed three times with PBS and fixed with 4% paraformaldehyde for 20 min at RT for imaging. For GFP imaging, the 488 nm line of an argon laser was used as excitation, and emission was collected using a BP 505–550 filter. The uptake experiments were visualized using a LSM 710 confocal microscope (Carl Zeiss, Oberkochen) and a Plan-Apochromat 63×/1.40 oil lens. MDA-MB-231 cells were transfected with Alexa 456 fluorescently labeled nanoconstructs. The next day, cells were washed three times with PBS and fixed with 4% paraformaldehyde for 20 min at RT for imaging. For Alexa 546 imaging, a DPSS 561 laser was used for excitation, and emission was collected between 566 and 680 nm.

Benjes C.M, & Brown J.F. (2001). Database-generated Web pages: the Norris Medical Library experience. Bulletin of the Medical Library Association, 89(2), 222-224.

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