The protein extraction kit (KeyGEN, China) was used to extract the cells or tissue proteins. The following protein antibodies were labeled: rabbit anti-mouse TGF-βr2 or TGF-β2 (1:500, eBioscience, USA), rabbit anti-mouse p-TGF-βr2 (1:200, Bioss, China), rabbit anti-mouse Smad2/3 (1:500, ThermoFisher, USA), mouse anti-mouse p-Smad2/3 (1:500, ThermoFisher, USA), mouse anti-mouse GAPDH (1:4000, ThermoFisher, USA). After being washed for three times, the membranes were incubated with the following secondary antibodies: HRP conjugated anti-rabbit IgG (1:5000, Fudebio, China) or HRP conjugated anti-mouse IgG (1:5000, CST, USA). The protein bands were exposured by the ECL detection system (Protein Simple, USA) and analyzed by ImageJ v1.42 software (National Institutes of Health, USA).
Mouse anti mouse gapdh
Manufactured by Thermo Fisher Scientific
Sourced in United States
Mouse anti-mouse GAPDH is a monoclonal antibody that specifically recognizes the mouse glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. GAPDH is a ubiquitous enzyme involved in glycolysis and is commonly used as a control for normalizing gene and protein expression in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Ripa buffer, by Thermo Fisher Scientific (2 mentions)
Protein extraction kit, by Keygen Biotech (1 mentions)
Phosphatase inhibitor, by Merck Group (1 mentions)
Pvdf blotting membrane, by GE Healthcare (1 mentions)
Goat anti rabbit hrp, by Southern Biotech (1 mentions)
Rabbit anti mouse akt, by Cell Signaling Technology (1 mentions)
Rabbit anti mouse pakt ser473, by Cell Signaling Technology (1 mentions)
Protease inhibitor cocktail, by Roche (1 mentions)
2 protocols using mouse anti mouse gapdh
1
Protein Expression Analysis via Western Blot
2
Western Blot Analysis of Insulin Signaling
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Tissues were homogenized using RIPA buffer (Thermo Fisher Scientific) and freshly added protease inhibitor cocktail (Roche) and phosphatase inhibitor (Sigma). Lysates of gWAT (20 µg), liver (40 µg), and skeletal muscle (40 µg) of db/db mice and of liver (30 µg) from Lsd1 DL db/db mice were analyzed by SDS-PAGE on 12% Bis-Tris gels with equal amount of protein loading.
Proteins were visualized after transfer onto a PVDF blotting membrane (GE Healthcare) and incubation with specific primary antibodies using horseradish peroxidase-conjugated secondary antibodies. Western blot primary antibodies included: rabbit anti-mouse Akt (Cell Signaling Technology, #4691S), rabbit anti-mouse p-Akt Ser473 (Cell Signaling Technology, #4060S), mouse anti-mouse GAPDH (Thermo Fisher, #AM4300), rabbit-anti-mouse LSD1 (Abcam, #17721) and mouse anti-mouse Vinculin (Abcam, #ab18058). Western blot secondary antibodies included: goat anti-rabbit HRP (Southern Biotech, #4010-05) and ECL sheep anti-mouse (GE Healthcare #NA931V).
Proteins were visualized after transfer onto a PVDF blotting membrane (GE Healthcare) and incubation with specific primary antibodies using horseradish peroxidase-conjugated secondary antibodies. Western blot primary antibodies included: rabbit anti-mouse Akt (Cell Signaling Technology, #4691S), rabbit anti-mouse p-Akt Ser473 (Cell Signaling Technology, #4060S), mouse anti-mouse GAPDH (Thermo Fisher, #AM4300), rabbit-anti-mouse LSD1 (Abcam, #17721) and mouse anti-mouse Vinculin (Abcam, #ab18058). Western blot secondary antibodies included: goat anti-rabbit HRP (Southern Biotech, #4010-05) and ECL sheep anti-mouse (GE Healthcare #NA931V).
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