Fxcycl pi rnase staining solution

Manufactured by Thermo Fisher Scientific

Sourced in United States

The FxCycl PI/RNase staining solution is a lab equipment product designed for cell cycle analysis. It contains propidium iodide (PI) and RNase, which are used to stain cellular DNA and remove RNA, respectively, enabling the determination of DNA content and cell cycle phase.

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Lab products found in correlation

Fcs express 6, by De Novo Software (1 mentions) Guava easycyte ht, by Merck Group (1 mentions)

Close spelling variants of this product

PI/RNase Staining Solution PI/RNase solution PI staining solution PI/RNase RNAse solution PI solution

2 protocols using fxcycl pi rnase staining solution

Cell Cycle Analysis of Prostate Cancer Cell Lines

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RWPE-2, PC3, DU145, and MDA-PCa-2b cells were serum-starved overnight and transfected with NC or miR-214 mimic for 48 h and then harvested by trypsinization. Cells were washed in ice-cold phosphate-buffered saline (PBS) and fixed in ice-cold 70% ethanol overnight at −20 °C. Cell pellets were stained with FxCycl PI/RNase staining solution (Life Technologies Corporation) for 15 min at room temperature in the dark. Multicycle DNA cell cycle analyses were performed with a Cytoflex flow cytometer using FCS Express-6 software (De Novo Software, Glendale, CA). Data from 10,000 gated events/samples were collected for each data file, and the experiments were repeated three times.

Cagle P., Niture S., Srivastava A., Ramalinga M., Aqeel R., Rios-Colon L., Chimeh U., Suy S., Collins S.P., Dahiya R, & Kumar D. (2019). MicroRNA-214 targets PTK6 to inhibit tumorigenic potential and increase drug sensitivity of prostate cancer cells. Scientific Reports, 9, 9776.

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Cell Cycle Analysis by Flow Cytometry

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The cell cycle was analyzed using FxCycl PI/RNase Staining Solution from Life Technologies (Carlsbad, CA, USA). Cells were harvested and fixed with 70% ethanol at 4 °C overnight; then cells were washed and stained with 0.5 mL of FxCycle PI/RNase Staining Solution for 30 min at room temperature in the dark. Finally, the cell cycle was measured using a flow cytometer (Guava EasyCyte HT, EMD Millipore, Billerica, MA, USA), and the data were analyzed using Flow Jo software (FLOWJO, Ashland OR, USA).

Ai J., Li J., Su Q., Ma H., He R., Wei Q., Li H, & Gao G. (2021). rAAV-based and intraprostatically delivered miR-34a therapeutics for efficient inhibition of prostate cancer progression. Gene therapy, 29(7-8), 418-424.

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