Fitc conjugated anti human cxcr5 mab

Manufactured by Miltenyi Biotec

Sourced in Germany

The FITC-conjugated anti-human CXCR5 mAb is a monoclonal antibody that binds to the CXCR5 receptor on human cells. It is conjugated with the fluorescent dye FITC, which allows for the detection and analysis of CXCR5-expressing cells using flow cytometry.

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Lab products found in correlation

Anti fitc microbeads, by Miltenyi Biotec (2 mentions)

2 protocols using fitc conjugated anti human cxcr5 mab

Isolation and Purification of CXCR5+ CD4+ T Cells

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Peripheral blood mononuclear cells (PBMCs) were isolated by density-gradient centrifugation with Ficoll-Hypaque solution (Haoyang Biological Technology Co, Tianjin, China). CD4⁺ T cells were isolated from PBMCs through immunomagnetic cell sorting using negative selection kits, and CXCR5⁺ cells were purified from CD4⁺ T cells by FITC-conjugated anti-human CXCR5 mAb and anti-FITC microbeads (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) according to the manufacturer’s instructions.

Yang J., Geng L., Ma Y., Tang X., Peng H., Tian J., Xu H, & Wang S. (2021). SLAMs Negatively Regulate IL-21 Production in Tfh-Like Cells from Allergic Rhinitis Patients. Journal of Asthma and Allergy, 14, 361-369.

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Isolation of CXCR5+ CD4+ T cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peripheral blood mononuclear cells (PBMCs) were isolated by density-gradient centrifugation with Ficoll-Hypaque solution (Haoyang Biological Technology Co., Tianjin, China). CD4 + T cells were isolated from PBMCs through immunomagnetic cell sorting using negative selection kits, and CXCR5 + cells were puri ed from CD4 + T cells by FITC-conjugated anti-human CXCR5 mAb and anti-FITC microbeads (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) according to the manufacturer's instructions.

Yang J., Geng L., Ma Y., Tang X., Peng H., Tian J., Xu H., & Wang S. (2020). SLAM Negatively Regulates IL-21 Production in Tfh-Like Cells from Allergic Rhinitis Patients.

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