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Serpinb3 antibody

Manufactured by Thermo Fisher Scientific
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The SerpinB3 antibody is a laboratory reagent used for the detection and quantification of the SerpinB3 protein in various biological samples. SerpinB3 is a member of the serpin (serine protease inhibitor) family and plays a role in regulating cellular processes. The antibody can be used in techniques such as Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA) to measure SerpinB3 levels.

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Lab products found in correlation

Background sniper, by Biocare Medical (2 mentions) Mach 4 universal hrp polymer, by Biocare Medical (2 mentions) Ph6 retrieval buffer, by Biocare Medical (2 mentions) Betazoid dab chromogen kit, by Biocare Medical (2 mentions) Peroxidase 1, by Biocare Medical (2 mentions)

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SERPINB3 (2 citations)

2 protocols using serpinb3 antibody

1

Immunohistochemical Analysis of IDH-wild-type GBM

Check if the same lab product or an alternative is used in the 5 most similar protocols
Standard immunohistochemistry analysis was performed on two patient specimens with a diagnosis of primary IDH-wild-type GBM using SerpinB3 antibody (Invitrogen, PA5–30164) diluted at 1:1000. Four-micrometer thick sections of FFPE tissue on charged slides were baked in the oven at 60C for 60 min before being deparrafinized and re-hydrated. Antigen retrieval was achieved using a pH6 retrieval buffer (Biocare Reveal). Slides were cooled to room temperature and washed in TBS before neutralizing endogenous peroxidase (Biocare Peroxidase 1). Slides were then treated with a serum-free casein background block (Biocare Background Sniper) before pre-incubation in a 10% goat serum block for 60 min. Primary antibody was then added to the slides for overnight incubation at 4C. After incubation, slides were washed well with TBS-T before incubating in HRP polymer (Biocare MACH 4 Universal HRP Polymer). Finally, reaction products were visualized with DAB (Biocare Betazoid DAB Chromogen Kit). Slides were then counterstained with hematoxylin, dehydrated and mounted with xylene-based mounting media.
Lauko A., Volovetz J., Turaga S.M., Bayik D., Silver D.J., Mitchell K., Mulkearns-Hubert E.E., Watson D.C., Desai K., Midha M., Hao J., McCortney K., Steffens A., Naik U., Ahluwalia M.S., Bao S., Horbinski C., Yu J.S, & Lathia J.D. (2022). SerpinB3 drives cancer stem cell survival in glioblastoma. Cell reports, 40(11), 111348.
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2

Immunohistochemical Analysis of IDH-wild-type GBM

Check if the same lab product or an alternative is used in the 5 most similar protocols
Standard immunohistochemistry analysis was performed on two patient specimens with a diagnosis of primary IDH-wild-type GBM using SerpinB3 antibody (Invitrogen, PA5–30164) diluted at 1:1000. Four-micrometer thick sections of FFPE tissue on charged slides were baked in the oven at 60C for 60 min before being deparrafinized and re-hydrated. Antigen retrieval was achieved using a pH6 retrieval buffer (Biocare Reveal). Slides were cooled to room temperature and washed in TBS before neutralizing endogenous peroxidase (Biocare Peroxidase 1). Slides were then treated with a serum-free casein background block (Biocare Background Sniper) before pre-incubation in a 10% goat serum block for 60 min. Primary antibody was then added to the slides for overnight incubation at 4C. After incubation, slides were washed well with TBS-T before incubating in HRP polymer (Biocare MACH 4 Universal HRP Polymer). Finally, reaction products were visualized with DAB (Biocare Betazoid DAB Chromogen Kit). Slides were then counterstained with hematoxylin, dehydrated and mounted with xylene-based mounting media.
Lauko A., Volovetz J., Turaga S.M., Bayik D., Silver D.J., Mitchell K., Mulkearns-Hubert E.E., Watson D.C., Desai K., Midha M., Hao J., McCortney K., Steffens A., Naik U., Ahluwalia M.S., Bao S., Horbinski C., Yu J.S, & Lathia J.D. (2022). SerpinB3 drives cancer stem cell survival in glioblastoma. Cell reports, 40(11), 111348.
+ Open protocol
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