Quantum ls imaging energy filter

Manufactured by Ametek

The Quantum-LS imaging energy filter is a laboratory equipment designed to select and filter specific energy levels within a sample. Its core function is to enable high-resolution energy-filtered imaging and spectroscopy.

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Lab products found in correlation

Fei titan krios, by Thermo Fisher Scientific (1 mentions) K2 summit, by Ametek (1 mentions) Vitrobot mark 4 plunger, by Thermo Fisher Scientific (1 mentions) Titan krios g3 electron microscope, by Thermo Fisher Scientific (1 mentions) K2 summit camera, by Ametek (1 mentions)

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Quantum energy filter (41 citations) Quantum LS (6 citations) Imaging Filter (5 citations) Quantum LS imaging filter (4 citations) Imaging Filter “Quantum” (4 citations) Quantum-LS Energy Filter (2 citations) Gatan Quantum-LS imaging energy filter (2 citations)

2 protocols using quantum ls imaging energy filter

Cryo-EM Data Acquisition on FEI Titan Krios

Cited 2 times

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Cryo-EM data were collected on a FEI Titan Krios (Thermo Fisher Scientific) transmission electron microscope, operated at 300 kV and equipped with a Quantum-LS imaging energy filter (GIF, 20 eV zero loss energy window; Gatan Inc.) and a K2 Summit direct electron detector (Gatan Inc.) operated in dose fractionation mode. Data acquisition was controlled by the SerialEM¹⁵ software, performed in counting mode, with a 42 e⁻/Å² total exposure fractioned into 40 frames over 8 s. The physical pixel size was 0.639 Å at the sample level. The data were pre-processed via the FOCUS package^{38 (link)}, including drift-correction and dose-weighting using MotionCor2^{39 (link)} (grouping every 5 frames and using 3 × 3 tiles) and CTF estimation using CTFFIND4^{40 (link)} (using information between 30 Å and 5 Å from the movie stacks). With these settings, we collected two datasets: one using beam-image shift^{14 (link)}, with three shots per grid hole, comprising 2243 movies, and a second one taking a single shot per hole, with 2252 movies. Detailed data collection information are given in the Supplementary Table S1.

Righetto R.D., Anton L., Adaixo R., Jakob R.P., Zivanov J., Mahi M.A., Ringler P., Schwede T., Maier T, & Stahlberg H. (2020). High-resolution cryo-EM structure of urease from the pathogen Yersinia enterocolitica. Nature Communications, 11, 5101.

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Cryo-EM Structure Determination of PTCH1 and SHH-PTCH1 Complex

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Apo-PTCH1 nanodisc and GAS1–SHH–PTCH1 complex nanodisc samples were concentrated to ~1 mg/mL. A sample of 3 μL was loaded onto a glow-discharged holey carbon grid (Quantifoil R1.2/1.3, copper, 300 mesh). All the grids were blotted for 4 s at 16 °C and 100% relative humidity using an FEI Vitrobot Mark IV plunger before being plunge-frozen in liquid nitrogen–cooled liquid ethane. Cryo-EM data were collected using a Titan Krios G3 electron microscope (Thermo Fisher Scientific) operated at 300kV. Micrographs were acquired at the nominal magnification of 130,000x (calibrated pixel size of 1.06 Å on the sample level) using a K2 Summit camera (Gatan) equipped with a Quantum LS imaging energy filter (Gatan) with the slit width set at 20 eV. The dose rate on the camera was set to 8 e‒/pixel/s. The total exposure time for each micrograph was 10 s fractionated into 50 frames with 0.2-s exposure time for each frame. The data collection was automated using the Leginon software package (Suloway et al., 2005 (link)). A total of 26,139 micrographs from 3 data collection sessions were collected for the apo-PTCH1 sample, and a total of 5,571 micrographs were collected from 2 data collection sessions for the GAS1–SHH–PTCH1 complex sample.

Huang P., Wierbowski B.M., Lian T., Chan C., García-Linares S., Jiang J, & Salic A. (2022). Structural basis for catalyzed assembly of the Sonic hedgehog–Patched1 signaling complex. Developmental cell, 57(5), 670-685.e8.

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