Phospho ser144 pak antibody

Manufactured by Cell Signaling Technology

The Phospho-Ser144 PAK antibody is a research-use-only product designed to detect phosphorylation at Serine 144 of the p21-activated kinase (PAK) protein. PAK proteins are involved in various cellular processes, including cytoskeleton organization and cell motility. This antibody can be used to analyze the phosphorylation status of PAK in different experimental conditions.

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Lab products found in correlation

Odyssey system, by LI COR (1 mentions) Alexa fluor 488 anti chicken, by Thermo Fisher Scientific (1 mentions) Hoechst, by Merck Group (1 mentions) Anti map2, by Santa Cruz Biotechnology (1 mentions) Chicken anti gfp, by Aves Labs (1 mentions) Anti mouse alexa fluor 633, by Thermo Fisher Scientific (1 mentions) Rac1 antibody, by BD (1 mentions) Phalloidin tritc, by Merck Group (1 mentions) Pak1 antibody, by Santa Cruz Biotechnology (1 mentions)

2 protocols using phospho ser144 pak antibody

Monitoring PAK1 Phosphorylation by TRIO

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HEK293T cells were co-transfected with the indicated pbioGFP-TRIO variants and a pRK5-PAK1 plasmid. 48 h after transfection, cells were lysed in lysis buffer, and amounts of phosphorylated PAK1 were monitored by immunoblot analysis with a phospho-Ser144 PAK antibody (# 2606S, Cell Signaling) and a monoclonal PAK1 antibody (sc-166887). Total TRIO expression was detected with a GFP antibody (Torrey Pines Biolabs, #TP401). Immunoblot detections and band-intensity quantifications were made with the Odyssey system from Li-COR Biosciences.

Barbosa S., Greville-Heygate S., Bonnet M., Godwin A., Fagotto-Kaufmann C., Kajava A.V., Laouteouet D., Mawby R., Wai H.A., Dingemans A.J., Hehir-Kwa J., Willems M., Capri Y., Mehta S.G., Cox H., Goudie D., Vansenne F., Turnpenny P., Vincent M., Cogné B., Lesca G., Hertecant J., Rodriguez D., Keren B., Burglen L., Gérard M., Putoux A., Cantagrel V., Siquier-Pernet K., Rio M., Banka S., Sarkar A., Steeves M., Parker M., Clement E., Moutton S., Tran Mau-Them F., Piton A., de Vries B.B., Guille M., Debant A., Schmidt S, & Baralle D. (2020). Opposite Modulation of RAC1 by Mutations in TRIO Is Associated with Distinct, Domain-Specific Neurodevelopmental Disorders. American Journal of Human Genetics, 106(3), 338-355.

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Phospho-PAK Quantification in Cell Lines

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List of all primary antibodies used: for immunoblotting, phospho-Ser144 PAK antibody (rabbit; Cell Signaling, #2606S, 1/1000), PAK1 antibody (mouse; Santa Cruz sc-166887, 1/1000), GFP antibody (rabbit; Torrey Pines Biolabs, #TP401, 1/3000), RAC1 antibody (mouse; BD Biosciences 610651, 1/1000); for immunocytochemistry, anti-GFP (chicken, Aves Labs, 1/1000), anti-MAP-2 (Santa Cruz, 1/500). List of all secondary antibodies used: Dylight Rabbit 680 (Thermo Fisher Scientific, 35568), Dylight Mouse 800 (Thermo Fisher Scientific, SA5-35521), Alexa Fluor 488 anti-chicken (Thermo Fisher Scientific, A11039), Alexa Fluor 633 anti-mouse (Thermo Fisher Scientific, A21050), Phalloidin TRITC (Sigma-Aldrich, P1951, 1/40,000), Hoechst (Sigma-Aldrich, B2261, 1/50,000).
Cell culture, transfection and immunoblot analysis of Phospho-PAK amounts HEK293T and N1E-115 neuroblastoma cell lines were obtained from ATCC and were regularly tested for mycoplasma contamination by PCR. Cells were cultured and transfected as described in [25] . Immunoblot analysis for quantification of Phospho-PAK levels were also performed as described in [25] , as was the quantification of lamellipodia in N1E-115 cells.

Bonnet M., Roche F., Fagotto-Kaufmann C., Gazdagh G., Truong I., Comunale F., Barbosa S., Bonhomme M., Nafati N., Hunt D., Rodriguez M.P., Chaudhry A., Shears D., Madruga M., Vansenne F., Curie A., Kajava A.V., Baralle D., Fassier C., Debant A, & Schmidt S. (2023). Pathogenic TRIO variants associated with neurodevelopmental disorders perturb the molecular regulation of TRIO and axon pathfinding in vivo. Molecular psychiatry, 28(4).

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