Brca1 a8x9f

Manufactured by Cell Signaling Technology

BRCA1-A8X9F is a laboratory reagent that can be used to detect and analyze the BRCA1 protein in biological samples. It is a monoclonal antibody that specifically binds to the BRCA1 protein. The core function of this product is to enable researchers to study the expression and localization of the BRCA1 protein in cells and tissues.

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Lab products found in correlation

Nupage minigels, by Thermo Fisher Scientific (1 mentions) β actin, by Merck Group (1 mentions) Niraparib, by Selleck Chemicals (1 mentions) Rucaparib, by Selleck Chemicals (1 mentions) Dasatinib, by Selleck Chemicals (1 mentions) Blasticidin, by Thermo Fisher Scientific (1 mentions) Puromycin, by Thermo Fisher Scientific (1 mentions) Phospho histone h2a x ser139, by Cell Signaling Technology (1 mentions) 4336 bpc 100, by Bio-Techne (1 mentions) Anti cancer compound library, by Selleck Chemicals (1 mentions) Flag d6w5b, by Cell Signaling Technology (1 mentions) Klf4 h180, by Santa Cruz Biotechnology (1 mentions) Cleaved parp asp214 d64e10, by Cell Signaling Technology (1 mentions) Myc tag 9e10, by Santa Cruz Biotechnology (1 mentions) Ha f 7, by Santa Cruz Biotechnology (1 mentions) Klf4 d1f2, by Cell Signaling Technology (1 mentions) Abt 263, by Selleck Chemicals (1 mentions) Olaparib, by Selleck Chemicals (1 mentions) Anti flag m2 affinity gel, by Merck Group (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions)

2 protocols using brca1 a8x9f

Protein Analysis by SDS-PAGE and Western Blot

Cited 1 time

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Protein fractions were analyzed by SDS-PAGE followed by either Coomassie blue staining or Western blotting. Proteins were separated on 3 to 8% tris-acetate NuPAGE mini gels (Thermo Fisher Scientific) and stained by SimplyBlue SafeStain solutions (Invitrogen) for 60 min. Gels were washed with deionized water for 60 min and then 3% NaCl for an additional 2 hours—overnight to achieve maximum sensitivity. Western blots were performed as described previously (16 (link)). The following primary antibodies were used in our analysis: BRCA1-C20 (SCBT, sc-642; α-BRCT), BRCA1-Ab1 (Calbiochem, OP92; α-RING), BRCA1-A8X9F (Cell Signaling Technology, #14823; α-RING), BARD1 (SCBT, sc-11438), ubiquitin-pAb (Enzo Life Sciences, ADI-SPA-200), RAD51 (SCBT, sc-8349), and β-actin (Sigma-Aldrich, A5441).

Liang Y., Dearnaley W.J., Varano A.C., Winton C.E., Gilmore B.L., Alden N.A., Sheng Z, & Kelly D.F. (2017). Structural analysis of BRCA1 reveals modification hotspot. Science Advances, 3(9), e1701386.

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Antibody-based Protein Analysis Protocol

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Specific antibodies against KLF4 (D1F2, 1:1,000), PARP (46D11, 1:1,000), Cleaved PARP (Asp214) (D64E10, 1:1,000), FLAG (D6W5B, 1:1,000), Phospho‐Histone H2A.X (Ser139, 1:1,000), 53BP1 (P550, 1:1,000), and BRCA1 (A8X9F, 1:1,000) were purchased from cell signaling (Beverly, MA). KLF4 (H‐180, 1:1,000), KLF4 (F‐8, 1:1,000), PARP1 (H‐300, 1:1,000), HA (F‐7, 1:1,000), and Myc tag (9E10, 1:1,000) were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Antibodies against β‐actin (AC‐15, 1:5,000) and FLAG (M2, 1:2,000) were from Sigma‐Aldrich. Antibodies against PAR were purchased from Trevigen (4336‐BPC‐100, 1:1,000). The anti‐FLAG M2 affinity gel was from Sigma‐Aldrich. The PARP1 inhibitor niraparib, olaparib, and rucaparib were purchased from Selleckchem (Houston, TA). Puromycin and blasticidin were from Invitrogen. Cycloheximide was from Sigma. The anti‐cancer compound library was purchased from Selleckchem. The compounds ABT‐263 and dasatinib used in animal model were purchased from Selleckchem.

Zhou Z., Huang F., Shrivastava I., Zhu R., Luo A., Hottiger M., Bahar I., Liu Z., Cristofanilli M, & Wan Y. (2020). New insight into the significance of KLF4 PARylation in genome stability, carcinogenesis, and therapy. EMBO Molecular Medicine, 12(12), e12391.

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