Retinal cells on coverslips (see below) were fixed in 4% paraformaldehyde for 15 minutes, washed with 1XPBS and incubated in blocking buffer (see above) for 30 minutes at room temperature. Coverslips were incubated with primary antibodies overnight at 4C at the following concentrations: anti-rhodopsin 1:150 (EMD Millipore), anti-S-opsin 1:300 (Santa Cruz Biotechnology), anti-MAP2 1:1000 (Novocastra Laboratories), anti-TUJ1 1:500 (Neuromics), anti-Chx10 1:200 (Santa Cruz Biotechnology), anti-RBPMS 1:1000 (EMD Millipore), anti-Thy1 1:2000 (Abcam), anti glutamine synthetase 1:250 (Abcam), anti-AP2 1:100 (Abcam), anti-mCherry 1:1000 (Thermo Fisher), anti-Nr2e3 1:100 (a gift from Jeremy Nathans at Johns Hopkins), anti-recoverin 1:300 (EMD Millipore), anti-Ryk 1:150 (Abgent), and anti-Ryk 1:150 (a gift from Yimin Zou at UCSD). Stained coverslips were mounted in DAPI Fluoromount-G (SouthernBiotech).
Anti thy1
Manufactured by Abcam
Sourced in United Kingdom
Anti-Thy1 is a primary antibody that specifically recognizes the Thy1 (CD90) cell surface antigen. Thy1 is a glycosylphosphatidylinositol (GPI)-anchored protein that is expressed on the surface of various cell types, including T cells, neurons, and mesenchymal stem cells. This antibody can be used for the identification and characterization of Thy1-positive cells in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti ryk, by Abcepta (1 mentions)
Anti chx10, by Santa Cruz Biotechnology (1 mentions)
Anti tuj1, by Neuromics (1 mentions)
Anti glutamine synthetase, by Abcam (1 mentions)
Dapi fluoromount g, by Southern Biotech (1 mentions)
Anti mcherry, by Thermo Fisher Scientific (1 mentions)
Anti ap2, by Abcam (1 mentions)
Glutamate, by Merck Group (1 mentions)
Anti caspase 3, by Abcam (1 mentions)
Akt1 2, by Merck Group (1 mentions)
Anti nf κb p65, by Cell Signaling Technology (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Rapamycin, by Merck Group (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Cellquest software, by BD (1 mentions)
Facs caliber flow cytometer, by BD (1 mentions)
4 protocols using anti thy1
1
Immunohistochemical Staining of Retinal Cells
2
Investigating Neuroinflammatory Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rapamycin, glutamate, lipopolysaccharides (LPS) and an Akt inhibitor (Akt-1/2) were purchased from Sigma (St. Louis, MO). Antibodies included anti-Caspase-3, anti-Thy-1, (abcam, Hong Kong, China), anti-Akt (Thr308 and Ser473), anti-NF-κB p65, anti-I kappa B-alpha (IκB-α), anti-induced nitric oxide synthase (iNOS), anti-β-actin (Cell Signaling Technology, Inc., Danvers, MA) and anti-ionized calcium-binding adapter molecule (Iba) 1 (woka, Chuo-Ku, Osaka Japan).
3
Mesenchymal Stem Cell Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At P3, MSCs were characterized by using fluorescent-labeled monoclonal antibodies (mAb) for CD90, CD44, and CD34 surface markers. Adherent cells were trypsinized, washed with PBS, and incubated, at room temperature for 30 min in dark, with monoclonal allophycocyanin-conjugated antibody for CD90 (Anti-Thy1.1) (Abcam, Cambridge, UK), monoclonal phycoerythrin- (PE-) conjugated antibody for CD44 (Abcam, Cambridge, UK), and monoclonal PE-conjugated antibody for CD34 (Abcam, Cambridge, UK). Subsequently, cells were washed thrice with PBS and resuspended in 500 μl FACS buffer. Immunofluorescence on the viable cells was performed using BD FACS Calibur flow cytometer equipped with Cell Quest software (Becton Dickinson, New Jersey, USA) [7 (link)].
4
Multimarker Characterization of Cultured Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were characterized using fluorescent-labelled monoclonal antibodies (mAb) for CD45, CD90, and OCT4 markers. Cells at passages 3–5 were trypsinized with 0.25% trypsin–EDTA solution, washed with phosphate-buffered saline (PBS), counted and incubated at room temperature for 30 min in the dark, with monoclonal phycoerythrin (PE)-conjugated antibody for CD45 (Abcam, ab23396, UK), monoclonal fluorescein isothiocyanate (FITC)-conjugated antibody for CD90 (Anti-Thy1.1) (Abcam, ab225, UK), and the monoclonal FITC-conjugated antibody for OCT4 antibody (Abcam, ab181557, UK). Immunofluorescence on the viable cells was analyzed using Becton Dickinson, FACS caliber flow cytometer equipped with Cell Quest software [12 (link), 14 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!