Pre coated silica gel 60 f254 tlc plates

1D and 2D NMR spectra were recorded using Bruker 400 MHz spectrometer. The chemical shifts (δ) are reported in parts per million (ppm) relative to tetramethylsilane (TMS δ = 0). The coupling constants (J) are given in Hz. Deuterated methanol (CD3OD) was used as solvent in the NMR experiments. Mass spectral data ESI-MS, were recorded on Agilent technology instrument Accurate Mass Q-ToF spectrometer. The material used for chromatographic separation was Sephadex LH-20. Pre-coated silica gel 60 F₂₅₄ TLC plates (Merck, Germany) were used for monitoring fractions and spots were detected with UV light (254 and 365 nm). Mueller Hinton (Agar and Broth) and Sabouraud Dextrose (Agar and Broth) were used for preparation of culture media for the antibacterial and antifungal activities respectively. Gentamicin, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, and quercetin were purchased from Sigma-Aldrich Co., USA. All solvents used were of analytical grade.

Analytical grade ethanol, n-hexane, ethyl acetate (EtOAc), n-butanol (n-BuOH) and methanol (MeOH) were obtained from Fisher Scientific, UK. Pre-coated Silica gel 60 F₂₅₄ TLC plates (Merck^®, Darmstadt, Germany) were used for the Thin-layer Chromatography (TLC) analysis. Sephadex^® LH 20 was purchased from Sigma–Aldrich (St. Louis, MO, USA). Silica gel 60 (0.063–0.200 mm; Merck^®, Darmstadt, Germany) was used for column chromatography. Silica gel 60 H (Merck^®, Darmstadt, Germany) was used for Vacuum Liquid Chromatography (VLC). Dimethyl sulfoxide (DMSO) and DMSO-d6 were obtained from Sigma-Aldrich, Portugal.