Quantikine human il 33

Murine IL-33 was quantified in the sera and periparasitic tissues of mice using the DuoSet Mouse IL-33 assay (R&D Systems, Bio-Techne, Lille, France). Protein extracts from tissues were diluted to 0.5 g/L of total protein before assay. Human IL-33 and ST2s were quantified in serum and/or liver tissues using the Quantikine Human IL-33 and Quantikine Human ST2/IL-33R assays (R&D Systems), following the manufacturer’s recommendations.

The medium from the confluent normal fibroblast and the CAF were sampled at 48 h after plating and centrifuged to remove the cell debris. SDF-1 and IL-33 levels in medium were assayed using the Quantikine Human IL-33 and SDF1 Immunoassay kit (R&D Systems, Abingdon, United Kingdom) according to the manufacturer’s instructions. The measured levels of IL-33 were expressed as picograms and SDF-1 as per 1 mg of protein in the cell lysate. The supernatants were collected and analyzed using ELISA, according to the manufacturer’s standard protocol. ELISAs were performed in duplicate on three separate occasions and the data were expressed as means ± SDs.