Vindesine

Calf brain tubulin was purified as described [68 ] and lyophilized for storage. The stathmin-like domain of RB3, the chicken TTL, and synthetic DarPIN protein preparations were done as described previously [8 (link),69 (link),70 (link)]. Paclitaxel (PTX) was from Alfa Aesar Chemical; Docetaxel (DTX) was kindly provided by Rhône Poulenc Rorer, Aventis (Schiltigheim, France); Cabazitaxel (CTX), Ixabepilone (EPO), Nocodazole (NOC), Colchicine (COL), Noscapine (NOS), Podophyllotoxin (PPT), Mebendazole (MBZ), Albendazole (ABZ), Febendazole (FBZ), Vinblastine (VBL), Vincristine (VCR), and Vindesine (VDS) were from Sigma-Aldrich (Saint Louis, MO, USA); Plinabulin (PLIN), Eribulin (ERIB), and Maytansine (MAYT) were from MedChemExpress. The compounds were diluted in 99.8% DMSO-d6 (Merck, Darmstadt, Germany) to a final concentration of 10 mM and stored at −80 °C.

Novartis Pharma generously provided Smac mimetic LCL161. Cytostatic drugs daunorubicin, doxorubicin, etoposide, idarubicin, topotecan, SN-38 (active metabolite of irinotecan), vinblastine, vincristine and vindesine were obtained from Sigma-Aldrich (Munich, Germany). NVP-TAE684 was purchased from Selleckchem (Munich, Germany). Working solutions were prepared by dilution of drugs with medium or ddH₂O to designated concentrations.