Quantikine human tgf beta 1

The concentration of PLT growth factors, i.e. platelet derived growth factor AB (PDGF-AB), vascular endothelial growth factor (VEGF), basic-fibroblast growth factor (bFGF) and transforming growth factor-β1 (TGF-β1, in the immunoreactive form) was evaluated in triplicate in each PI-PL and control PL preparation by using commercially available immunoenzymatic kits: PDGF-AB (Quantikine Human PDGF-AB, R&D Systems, Minneapolis, Minnesota, USA), VEGF (Quantikine Human VEGF, R&D System), bFGF (Quantikine Human FGF basic, R&D System), TGF-Beta (Quantikine Human TGF-Beta 1, R&D System).

Cord blood samples were obtained from the umbilical vein after cord clamping at birth. Plasma was separated from ethylenediaminetetraacetic acid-treated blood using centrifugation at 1400× g for 10 min at 4 °C. Serum was separated using centrifugation at 2000× g for 10 min at room temperature. Sample aliquots were immediately stored at −80 °C until assayed.
N-terminal precursor of B-type natriuretic peptide (NT-proBNP) and Troponin I concentrations was measured in plasma by electrochemiluminescence immunoassay using Siemens Atellica IM NT-proBNP and High Sensitivity Troponin I, respectively (Siemens Healthcare, Erlangen, Germany). Transforming growth factor β1 (TGFβ) was measured in serum by conventional ELISA assay Quantikine Human TGF-beta1 (R&D Systems, Minneapolis, MN, USA). Concentrations of placental growth factor (PlGF) and soluble fms-like tyrosine kinase-1 (sFlt1) were determined in serum by the fully automated Elecsys assays for sFlt-1 and PlGF on an electrochemiluminescence immunoassay platform Cobas analyzer (Roche Diagnostics, Mannheim, Germany). Concentrations of cord NT-proBNP, TGFβ, PlGF, and sFlt1 are presented as continuous variables (NT-proBNP, PlGF, and sFtl1 in pg/mL and TGFβ in ng/mL). Troponin I was treated as a dichotomous variable, and concentrations above the technical detection limit (>0.017 ng/mL) were considered positive [41 (link)].