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2 protocols using meropenem

1

High-Throughput Screening of Transporter Modulators

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Calcitriol, calcipotriol, MK571, epigallocatechin gallate (EGCG), hyperoside, LTC4, meropenem, mesalamine, nitazoxanide, and vincristine were purchased from Cayman Chemical (Ann Arbor, MI). AMP, ATP, doxorubicin, estradiol 17-(β-D-glucuronide) (E217βG), mitoxantrone, poly-D-lysine, thiazolyl blue tetrazolium bromide (MTT), and verapamil were procured from Sigma-Aldrich (St. Louis, MO). Apigenin and Ko143 were purchased from Tocris Bioscience (Avonmouth, Bristol, UK). Calcein-AM was obtained from Corning Life Sciences (Corning, NY). [6,7-3H]E217βG (49.9 Ci mmol−1) and [14, 15, 19, 20-3H]LTC4 (177.6 Ci mmol−1) were purchased from PerkinElmer (Waltham, MA).
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2

Bacterial membrane characterization protocol

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LB media, MacConkey agar, Colistin sulfate and sodium acetate were acquired from Carl Roth GmbH & Co. KG (Karlsruhe, Germany). PBS was obtained from Capricorn Scientific GmbH (Ebsdorfergrund, Germany). Ham's F12 medium was purchased from GE Healthcare Europe (Freiburg, Germany). Polymyxin B was supplied by Merck Millipore (Billerica, USA). Gentamicin was obtained from Gibco Life Technologies (Carlsbad, USA) and Meropenem from Cayman Chemicals (Ann Arbor, USA). Methanol was acquired from Merck KGaA (Darmstadt, Germany). Chloroform was purchased from Acros Organics (Geel, Belgium). LPS (Salmonella minnesota R595, TLR grade) was supplied by Enzo Life Sciences (Lausen, Austria). All lipids were obtained from Avanti® Polar Lipids Inc. (Birmingham, USA). All other applied chemicals were of analytical grade and acquired from commercial sources.
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