Radixin thr564 moesin thr558

Manufactured by Cell Signaling Technology

Radixin (Thr564)/Moesin (Thr558) is a lab equipment product that detects the phosphorylation of radixin at threonine 564 and moesin at threonine 558. These sites are known to be important for the regulation of the cytoskeleton and cell motility.

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Lab products found in correlation

Monoclonal mouse antibody to bin1 clone c99d against exon 17, by Merck Group (2 mentions) Hrp conjugated beta actin monoclonal antibody, by Proteintech (2 mentions) Topfluor tmr pi 4 5 p2, by Avanti Polar Lipids (2 mentions) Culture inserts 2 well for self insertion, by Ibidi (1 mentions) Brain l α phosphatidylinositol 4 5 bisphosphate, by Avanti Polar Lipids (1 mentions) Brain total lipid extract, by Avanti Polar Lipids (1 mentions) Alexa fluor 647, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Moesin (8 citations) Radixin (3 citations) Rabbit anti-phospho Ezrin (Thr567)/ Radixin (Thr564)/ Moesin (Thr558), (2 citations)

2 protocols using radixin thr564 moesin thr558

Phospholipid-mediated Membrane Interactions

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Natural and synthetic phospholipids, including POPS, POPC, POPE_, brain total lipid extract, brain L-α-phosphatidylinositol-4,5-bisphosphate, and TopFluor-TMR-PI(4,5)P₂ are from Avanti Polar Lipids. OG-DHPE and β-casein from bovine milk (>98% pure) were from Sigma-Aldrich. Alexa Fluor 647 and 488 Maleimide labeling kits are from Invitrogen. Culture-Inserts 2 Well for self-insertion were purchased from ibidi.
The following antibodies were used in this study: monoclonal mouse antibody to BIN1 (clone C99D against exon 17) from Millipore, polyclonal rabbit antibody to IRSp53 (BAIAP2) from Atlas Antibodies (HPA023310), HRP-conjugated beta-actin monoclonal antibody from Proteintech (HRP-60008), ezrin antibody from M. Arpin laboratory^{75 (link)}, monoclonal rabbit antibody to phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Cell Signaling (Cat. 3726). Atto390 phalloidin was from Sigma.

Picas L., André-Arpin C., Comunale F., Bousquet H., Tsai F.C., Rico F., Maiuri P., Pernier J., Bodin S., Nicot A.S., Laporte J., Bassereau P., Goud B., Gauthier-Rouvière C, & Miserey S. (2024). BIN1 regulates actin-membrane interactions during IRSp53-dependent filopodia formation. Communications Biology, 7, 549.

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Lipid and Protein Labeling for Cellular Imaging

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Natural and synthetic phospholipids, including POPS, POPC, POPE and TopFluor-TMR-PI(4,5)P2 are from Avanti Polar Lipids, Inc. Alexa Fluor 647 and 488 Maleimide labelling kits are from Invitrogen.
The following antibodies were used in this study: monoclonal mouse antibody to BIN1 (clone C99D against exon 17) from Millipore, polyclonal rabbit antibody to IRSp53 (BAIAP2) from Atlas Antibodies (HPA023310), HRP-conjugated beta actin monoclonal antibody from Proteintech (HRP-60008), ezrin antibody from M. Arpin laboratory (Algrain et al., 1993) (link), monoclonal rabbit antibody to phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Cell Signaling (Cat. 3726). Atto390 488 phalloidin was from Sigma.

Picas L., Comunale F., André-Arpin C., Bousquet H., Tsai F., Rico F., Maiuri P., Pernier J., Bodin S., Nicot A., Laporte J., Bassereau P., Goud B., Gauthier‐Rouvière C., & Miserey‐Lenkei S. (2022). BIN1 regulates actin-membrane interactions during IRSp53-dependent filopodia formation.

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