Nova 6000 pe150 platform

The three Sham group specimens, the three CLP group specimens, and the three Maresin1 group specimens were mixed, respectively, for single-cell RNA sequencing. Single-cell suspensions of lung tissue specimens were prepared according to OE Biotech Co., Ltd.’s advice [22 (link)]. The cells were then sorted using Miltenyi mouse CD45 magnetic beads according to the manufacturer’s recommendations and sequenced at a 1:1 ratio. According to the manufacturer’s guidelines, the single-cell libraries were constructed by 10× Genomics Chromium Single Cell 3ʹ Reagent Kits v3.1. The Illumina Nova 6000 PE150 platform was used to sequence the libraries.

Single cells from each sample were independently processed into single‐cell suspensions and library generations on a 10× Genomics system. The freshly prepared single‐cell suspension was adjusted to a cell concentration of 700–1200 cells µL⁻¹, and the machine and library construction were carried out according to the operation manual of 10× Genomics Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Catalog No. 1000268). The constructed library was subjected to high‐throughput sequencing using the Illumina Nova 6000 PE150 platform.