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5 iodotubercidin 5 itu

Manufactured by Cayman Chemical
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5-Iodotubercidin (5-Itu) is an adenosine kinase inhibitor. It is used in biochemical and cell biology research applications.

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Lab products found in correlation

Abt702 dihydrochloride, by Cayman Chemical (2 mentions) Ethanol, by Thermo Fisher Scientific (1 mentions) Bay 1816032, by MedChemExpress (1 mentions) 5 itu, by Thermo Fisher Scientific (1 mentions) Bay 1816032, by Merck Group (1 mentions) Zm447439, by Bio-Techne (1 mentions) Zm447439, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Acidic tyrode s solution, by Merck Group (1 mentions)

4 protocols using 5 iodotubercidin 5 itu

1

Inhibitors for Mouse Oocyte Meiosis

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All inhibitors used for the mouse oocytes are listed in Table S3. 5-Iodotubercidin (5-Itu; Cayman Chemicals; 0.5μM) was used to inhibit haspin kinase with ethanol (Fisher) as the control (solvent for 5-Itu). To disrupt Bub1 H2A phosphorylation, we added BAY-1816032 (8μM, MedChem Express); we used DMSO (Sigma) as control (solvent for BAY-1816032). We used ZM447439 (Tocris Bioscience, 5μM) as an Aurora B/C inhibitor and DMSO (Sigma) as a control (solvent for ZM447439). For all mouse meiosis I experiments, BAY-1816032 and ZM447439 were added at 0h when transferring oocytes to CZB, while 5-Itu was added after 5hrs from CZB oocyte transfer. For all mouse meiosis II experiments, all drugs were added at 11hrs. Oocytes were fixed 8hrs after CZB oocyte transfer for meiosis I experiments and after 15hrs for meiosis II experiments. We treated all meiosis I mouse oocytes with acidic Tyrode’s solution (Millipore Sigma) to remove the zone pellucida when imaging for Aurora kinase C, kinetochores, and DNA.
Cairo G., Greiwe C., Jung G.I., Blengini C., Schindler K, & Lacefield S. (2023). Distinct Aurora B pools at the inner centromere and kinetochore have different contributions to meiotic and mitotic chromosome segregation. bioRxiv.
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2

Inhibiting Nicotinamide Riboside Metabolism

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Two known inhibitors for AK, 5-Iodotubercidin (5-Itu) and ABT702 dihydrochloride were purchased from Cayman Chemical (#10010375) and Tocris Bioscience (#2372) respectively. To test if these compounds can interfere with the NRH-induced NAD+ concentrations increases, HEK293 and Neuro2a cells were treated with 1 mM NRH or NR for 6 hr, with or without the addition of either 26 nM 5-Itu or 20 nM ABT702. In lysates, NMNH production was assayed in the presence of escalating concentrations of 5-Itu or ABT702 in the presence of 2 mM NRH, 2 mM ATP and 5 mM MgCl2 in pH 9 phosphate buffer and incubated at 37°C for 30 min, then quenched by heating at 80°C for 2 min. The supernatants were injected onto HPLC for NMNH and NRH quantifications. Additionally, HepG2 and C2C12 cells were treated with 1 mM NRH, with or without either 26 nM 5-Itu or 20 nM ABT702 for 6 hr, and their cellular NAD levels were examined.
Yang Y., Zhang N., Zhang G, & Sauve A.A. (2020). NRH salvage and conversion to NAD+ requires NRH kinase activity by adenosine kinase. Nature metabolism, 2(4), 364-379.
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3

Inhibiting Nicotinamide Riboside Metabolism

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Two known inhibitors for AK, 5-Iodotubercidin (5-Itu) and ABT702 dihydrochloride were purchased from Cayman Chemical (#10010375) and Tocris Bioscience (#2372) respectively. To test if these compounds can interfere with the NRH-induced NAD+ concentrations increases, HEK293 and Neuro2a cells were treated with 1 mM NRH or NR for 6 hr, with or without the addition of either 26 nM 5-Itu or 20 nM ABT702. In lysates, NMNH production was assayed in the presence of escalating concentrations of 5-Itu or ABT702 in the presence of 2 mM NRH, 2 mM ATP and 5 mM MgCl2 in pH 9 phosphate buffer and incubated at 37°C for 30 min, then quenched by heating at 80°C for 2 min. The supernatants were injected onto HPLC for NMNH and NRH quantifications. Additionally, HepG2 and C2C12 cells were treated with 1 mM NRH, with or without either 26 nM 5-Itu or 20 nM ABT702 for 6 hr, and their cellular NAD levels were examined.
Yang Y., Zhang N., Zhang G, & Sauve A.A. (2020). NRH salvage and conversion to NAD+ requires NRH kinase activity by adenosine kinase. Nature metabolism, 2(4), 364-379.
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4

Inhibiting HASPIN during Oocyte Maturation

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HASPIN inhibitor 5-iodotubercidin (5-Itu) (Cayman Chemical, cat no. 10010375) was dissolved in 100% ethanol. Oocyte maturation medium was used to dilute the 5-Itu stock solution to obtain the desired working solution. Because ethanol is the vehicle in which 5-Itu was dissolved, the same volume of ethanol was added into the medium as a control during oocyte maturation.
Cao Z., Xu T., Tong X., Zhang D., Liu C., Wang Y., Gao D., Luo L., Zhang L., Li Y, & Zhang Y. (2019). HASPIN kinase mediates histone deacetylation to regulate oocyte meiotic maturation in pigs. Reproduction (Cambridge, England), 157(6).
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