Human breast cancer cells MCF7 and ZR75 cells were obtained from American-Type Culture Collection (ATCC, Manassas, VA) and maintained and passaged in our laboratory for less than six months in RPMI-1640 medium supplemented with 10% FBS (Hyclone Laboratories Ltd, Logan, UT). Generation and characterization of MCF7-PELP1#20 and #13 (33 (link)), ZR75-PELP1(33 (link)), MCF7-PELP1 cyto model cells (34 (link)) were earlier described. PELP1 and Raptor antibodies were purchased from Bethyl lab (Montgomery, TX). Ki-67 was from Dako (Carpinteria, CA) and all other antibodies were purchased from Cell Signaling Technology (Boston, MA). β-actin and all secondary antibodies were from Sigma Chemical Co (St. Louis, MO). PELP1 SMARTpool human siRNA was purchased from Dharmacon (Lafayette, CO). Rapamycin and AZD8055 were purchased from Tocris Bioscience (Ellisville, Mo). Captisol was purchased from CyDex (La Jolla, CA).
Azd8055
Manufactured by Bio-Techne
Sourced in United Kingdom
AZD8055 is a selective and potent inhibitor of the mammalian target of rapamycin (mTOR) kinase. It acts by blocking the activity of both mTOR complexes, mTORC1 and mTORC2.
Automatically generated - may contain errors
Lab products found in correlation
Rapamycin, by Bio-Techne (2 mentions)
Fetal bovine serum (fbs), by Cytiva (1 mentions)
Rpmi 1640 medium, by Cytiva (1 mentions)
β actin, by Merck Group (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Zr 75, by American Type Culture Collection (1 mentions)
Ouabain, by Bio-Techne (1 mentions)
Hydroxyurea, by Merck Group (1 mentions)
Wortmannin, by Bio-Techne (1 mentions)
Azd8055, by Santa Cruz Biotechnology (1 mentions)
Wortmannin, by Merck Group (1 mentions)
Ouabain, by Merck Group (1 mentions)
Withaferin a, by Bio-Techne (1 mentions)
Pik 90, by Merck Group (1 mentions)
Ly294002, by Bio-Techne (1 mentions)
Rapamycin, by Merck Group (1 mentions)
2 protocols using azd8055
1
Characterizing PELP1-driven breast cancer models
2
Modulation of Zebrafish Development
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Ouabain (Sigma-Aldrich; St. Louis, MO) and hydroxyurea (Merck, Kenilworth, NJ) were dissolved in water, Wortmannin (Sigma-Aldrich), PIK90 (Merck), LY294002 (TocrisBioscience, Bristol, UK), Rapamycin (Merck), AZD8055 (Santa Cruz Biotechnologies), and Withaferin A (TocrisBioscience) in DMSO, and solutions were further diluted in E3 embryo medium to concentrations of 3mM (Ouabain), 50mM (hydroxyurea), 1 µM (Wortmannin), 5 µM (PIK90), 25 µM (LY294002), 1.1 µM (Rapamycin), 30 µM (AZD8055), and 30 µM (WithaferinA). Embryos were incubated in inhibitor solution starting from 34 hpf and scored at 54 hpf.
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