Immunoblotting was performed using the following antibodies diluted in TBS supplemented with 0.1% Tween 20 and 5% milk powder or 3% BSA: cyclin E1 (1:1000, Santa Cruz, sc-247), beta-actin (1:1000, Santa Cruz, sc-81178), p-p53 S15 (1:1000, Cell Signalling, 9284), p-CHK1 S345 (1:1000, Cell Signalling, 2348), p-RPA S4/S8 (1:5000, Bethyl Laboratories, A300-245), HA.11 (1:1000, BioLegend, 16B12), p53 (1:1000, Santa Cruz, sc-126), p21 (1:1000, Cell Signalling, 2947), HA (1:1000, Santa Cruz, sc-805), alpha-Tubulin (1:4000, Sigma, T5168), POT1 (1:1000, Novus Biologicals, NB500-176), FBXW7 (1:1000, Proteintech, 55290-1-AP), p-CDK1 T14/Y15 (in-house by Julian Gannon), Cdh1 (1:1000, in-house by Julian Gannon, AR38.2), anti-Mouse HRP (1:5000, Dako, P0447) and anti-Rabbit HRP (1:5000, Jackson Immuno, 711-035-152). The following antibodies were used for FACS and diluted in PBS supplemented with 1% BSA: MCM7 (1:200, Santa Cruz, sc-56324), cyclin B1 (1:200, Abcam, ab32053), p-H2A.X S139 (1:200, Millipore, 05-636), anti-Rabbit Alexa Fluor 555 (1:500, Thermo, A21428) and anti-Mouse Alexa Fluor 555 (1:500, Thermo, A21424).
P rpa s4 s8
Manufactured by Fortis Life Sciences
The P-RPA S4/S8 is a piece of laboratory equipment designed for rapid purification and concentration of RNA. It utilizes a combination of magnetic bead-based technology and proprietary reagents to efficiently extract and purify RNA samples from a variety of biological sources.
Automatically generated - may contain errors
Lab products found in correlation
Anti rabbit alexafluor 555, by Thermo Fisher Scientific (2 mentions)
Cyclin b1, by Abcam (1 mentions)
Ha 11, by BioLegend (1 mentions)
Anti mouse alexa fluor 555, by Thermo Fisher Scientific (1 mentions)
Cyclin e1, by Santa Cruz Biotechnology (1 mentions)
Fbxw7, by Proteintech (1 mentions)
P h2a x s139, by Merck Group (1 mentions)
P p53 s15, by Cell Signaling Technology (1 mentions)
Pchk1 s345, by Cell Signaling Technology (1 mentions)
Alpha tubulin, by Merck Group (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
α tubulin, by Cell Signaling Technology (1 mentions)
2 protocols using p rpa s4 s8
1
Immunoblotting and FACS Analysis
2
Antibody-based Detection of DNA Damage Response Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A MERIT40 rabbit polyclonal antibody raised against mouse MERIT40 was generated against a GST-MERIT40 fusion protein and used at 1:200 for immunoblotting and 1:100 for immunofluorescence as previously described (Shao et al. 2009 (link)). γ-H2AX (Millipore, JBW301) was used at 1:2000 for immunofluorescence and 1:1000 for immunoblotting. BRCA1 was detected by immunofluorescence with a homemade rabbit polyclonal antibody raised against the exon 11 region of mouse BRCA1 at 1:100. Homemade Rap80 rabbit polyclonal antibody was used for immunoblotting at 1:500 and immunofluorescence at 1:150. BRCC36 was detected for immunoblotting with a rabbit polyclonal antibody (Sobhian et al. 2007 (link)) at 1:1000. KIAA0157 was detected with a rabbit polyclonal antibody (LSBio, LS-C102632) diluted at 1:1000 (Zheng et al. 2013 (link)). α-Tubulin (1:10,000; Cell Signaling, DM1A) was used as a loading control on immunoblots. p-RPA (S4/S8) (Bethyl Laboratories, A300-245a) was used for immunoblotting at 1:1000. Total RPA (Novus Biologicals, NB600-565) was used for immunoblotting at 1:200. FANCD2 (Abcam, ab108928) was used of immunoblotting at 1:200. RPA32/RPA2 (Abcam, ab61184) was used for immunofluorescence in mouse cells at 1:200.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!