NK cells and other lymphocyte subsets in patients' blood after NK infusions were quantified using Trucount tubes (BD Biosciences), BD Multitest six-color TBNK reagent (BD Biosciences) and FACSDiva software (BD Biosciences) according to the manufacturer's instructions. Donors' and patients' NK cells were distinguished and quantified by short tandem repeat chimerism analysis (AmpFlSTR Profiler Plus polymerase chain reaction amplification kit; Thermo Fisher Scientific, Waltham, MA, USA) with DNA isolated from NK cells purified using RoboSep (Stem Cell Technologies, Vancouver, Canada). The sensitivity of the chimerism assay was 1À2%.
Multitest six color tbnk reagent
Manufactured by BD
Sourced in United States
The BD Multitest six-color TBNK reagent is a laboratory equipment product used for the identification and enumeration of T cells, B cells, and natural killer cells in human whole blood samples. The reagent contains a combination of fluorescent-labeled monoclonal antibodies that bind to specific cell surface markers, allowing for the detection and quantification of these cell populations using flow cytometry.
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Lab products found in correlation
2 protocols using multitest six color tbnk reagent
1
Quantifying Immune Cell Dynamics after NK Infusions
2
CD4+ T Cell Enumeration by Flow Cytometry
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The proportion of CD4 + T cell (%CD4 + T cells) on FACSCanto II flow cytometry (BD Biosciences, San Jose, CA, USA) by analyzing 30,000 events by the lymphocyte gate was measured as a reference method using the BD Multitest six-color TBNK reagent (CD3 FITC/CD16 PE + CD56 PE/CD45 PerCP-Cy5.5/CD4 PE-Cy7/CD19 APC/CD8 APC-Cy7) [18 (link)]. The reference flow cytometry instrument was calibrated daily and checked by running Multicheck Normal and Low controls (BD Biosciences). The results were analyzed using FACS Diva software (BD Biosciences) for routine CD4 + T cell counting in a clinical laboratory.
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