Serum urea and creatinine were measured with spectrophotometric reagents commercially available (MAK006-1KT, MAK080-1KT, Sigma, Saint-Louis, MO, USA) and the absorbance was measured using Multiskan FC Microplate Photometer (Thermo Fisher Scientific, Waltham, MA, USA). Blood urea nitrogen (BUN) was calculated using the following formula: urea (mg/dL) × 0.467 = BUN (mg/dL).
Mak080 1kt
Manufactured by Merck Group
Sourced in United States
The MAK080-1KT is a lab equipment product from Merck Group. It is a detection kit that is used to measure the activity of the enzyme myeloperoxidase (MPO) in biological samples. The kit provides the necessary reagents and instructions to perform the MPO activity assay.
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2 protocols using mak080 1kt
1
Spectrophotometric Determination of Serum Urea and Creatinine
2
Serum Biomarkers for Kidney Injury
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Blood biochemical analysis was performed according to previous investigations. The blood samples were extracted 24 h following sham or CLP operation and centrifuged at 1400 x g to prepare serum at 4°C for 10 min. SCr levels were evaluated by a creatinine enzymatic assay kit (MAK080-1KT, Sigma-Aldrich, Merck KGaA) and measured at 570 nm. BUN was detected with an enzymatic assay kit (MAK006-1KT, Sigma-Aldrich, Merck KGaA) and measured at 570 nm. The expression levels of NGAL (ab119602, Abcam) and KIM-1(ab119597, Abcam) were measured using corresponding kits and measured at 450 nm. All measurements were conducted with a multiscan spectrum spectrophotometer (Thermo Fisher Scientific Inc.)
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