Total liver ubiquinone (CoQ9 and CoQ10) was quantified by reverse-phase HPLC with UV detection at 275 nm, as described previously (18 (link), 19 (link)). Serum was obtained as detailed previously (18 (link)), and blood from the tail vein collected into EDTA tubes and centrifuged for 3 min at 3000 rpm at 4° Celsius to isolate plasma. Fasted blood glucose measurements were obtained by using a glucose analyzer (Hemocue). The serum lipid profile and fasted plasma insulin analyses were performed by using an auto-analyzer (the Wellcome Trust–supported Cambridge Mouse Laboratory). Liver triglyceride concentrations were determined by using the Folch assay (23 ). Briefly, liver samples were homogenized in a 2:1 ratio of chloroform:methanol. The distinct lipid phase was removed after centrifugation, and lipid weight was quantified after the solvent was removed by evaporation.
Glucose analyzer
Manufactured by HemoCue
Sourced in Sweden
The Glucose Analyzer is a compact and portable device designed to measure glucose levels in blood samples. It utilizes advanced technology to provide accurate and reliable results. The Glucose Analyzer is intended for use in professional healthcare settings.
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Lab products found in correlation
2 protocols using glucose analyzer
1
Quantifying Liver Ubiquinone and Metabolic Markers
2
Blood Glucose Measurement Techniques
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The measurement of blood glucose (study 1 and 2) was performed immediately after collection using the glucose dehydrogenase method (GlucoseAnalyzer, HemoCue, Ängelholm, Sweden). For other analyses, blood samples were cold centrifuged at -4°C directly after collection and plasma was stored at -80°C. Glucagon levels were determined using an immunoassay, as described previously (Lilly Research Laboratories, Indianapolis, IN, USA) (Sloan et al. 2012) . Total GLP1 was analysed using a C-terminally directed radioimmunoassay for amidated GLP1 (antibody 89390 (Orskov et al. 1994) (link)). Insulin levels were measured using an immunometric assay (Advia, Centaur, Siemens Medical Solutions Diagnostics, Malvern, PA, USA). Total insulin, glucagon and GLP1 levels were only determined in study 1.
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