Cells were grown in LB at 37 °C until OD 600 = 0.5. Cells growing in exponential phase were spotted onto a 1% agarose phosphate-buffered saline pad for imaging. Cells were imaged on a Nikon Eclipse Ti2-E inverted fluorescence microscope with a CFI Plan Apochromat DM Lambda 100X Oil, N.A. 1.45, W.D. 0.13 mm objective. Images were collected on a Prime 95B 25 mm camera (Photometrics). We used a Cy5-4050C (32 mm) filter cube (Nikon). Image acquisition was performed with NIS-Element Advance Research version 4.5.
Cfi plan apochromat dm lambda 100x oil
Manufactured by Nikon
The CFI Plan Apochromat DM Lambda 100X Oil is a high-performance microscope objective lens designed for use in various scientific and laboratory applications. It features a magnification of 100X and is optimized for use with oil immersion. The lens is made using Nikon's advanced optical technologies to provide exceptional image quality and resolution.
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Lab products found in correlation
2 protocols using cfi plan apochromat dm lambda 100x oil
1
Fluorescence Microscopy of Bacterial Cells
2
Fluorescence Microscopy of Bacterial Cells
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Cells were grown in LB at 37 °C until OD 600 = 0.5. Cells growing in exponential phase were spotted onto a 1% agarose phosphate-buffered saline pad for imaging. Cells were imaged on a Nikon Eclipse Ti2-E inverted fluorescence microscope with a CFI Plan Apochromat DM Lambda 100X Oil, N.A. 1.45, W.D. 0.13 mm objective. Images were collected on a Prime 95B 25 mm camera (Photometrics). We used a Cy5-4050C (32 mm) filter cube (Nikon). Image acquisition was performed with NIS-Element Advance Research version 4.5.
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