Brain sections were blocked for 1 hour at room temperature with 5% BSA and incubated with primary antibodies mouse monoclonal anti-GFAP (1:1000; Millipore; Cat# MAB360, RRID:AB_11212597) and rabbit polyclonal anti-AQP4 (1:400; Millipore; Cat# AB3594, RRID:AB_91530) at 4°C overnight. The next day, all sections were rinsed three times and incubated for 2 hours at room temperature in a mixture of Alexa Flour 555 donkey anti-mouse IgG (1:1000; Thermo Fisher Scientific (China) Co., Ltd., Shanghai, China; Cat# A31570, RRID:AB_2536180) and Alexa Flour 488 donkey anti-rabbit IgG (1:1000; Thermo Fisher Scientific (China) Co., Ltd.; Cat# A21206, RRID:AB_2534073). After rinsing, the sections were incubated for 6 minutes in 4′,6-diamidino-2-phenylindole (1:1000; Thermo Fisher Scientific (China) Co., Ltd.; Cat# D1306) and coverslipped with anti-fluorescent quencher.
Alexa flour 555 donkey anti mouse igg
Manufactured by Thermo Fisher Scientific
Sourced in China
Alexa Fluor 555 donkey anti-mouse IgG is a secondary antibody that binds to mouse immunoglobulin G (IgG) and is conjugated with the Alexa Fluor 555 fluorescent dye. It is used in immunofluorescence and other applications where detection of mouse primary antibodies is required.
Automatically generated - may contain errors
3 protocols using alexa flour 555 donkey anti mouse igg
1
Immunofluorescence Staining of GFAP and AQP4
2
Immunofluorescent Labeling of GFAP and AQP4
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Frozen slices were blocked for 1 hour at room temperature with 5% BSA and then incubated with a mixture of primary antibodies including mouse anti‐GFAP (1:1000) and rabbit anti‐AQP4 (1:400) overnight at 4°C. Following PBS rinse, sections were incubated for 2 hours at room temperature with a mixture of Alexa Flour™ 555 donkey anti‐mouse IgG (1:1000; Thermo Fisher; Cat. # A31570) and Alexa Flour™ 488 donkey anti‐rabbit IgG (1:1000; Thermo Fisher; Cat. # A21206), followed by counterstaining with 4′, 6‐diamidino‐2‐phenylindole (DAPI, 1:1000; Thermo Fisher; Cat. # D21490) for 6 minutes and then cover‐slipped with antifluorescence quenching sealant.
3
Immunofluorescence and Thioflavine-S Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Brain sections were blocked for 1 hour at room temperature with 5% BSA and incubated with the primary antibodies including mouse anti-GFAP (1:1000) and rabbit anti-AQP4 (1:400) at 4 °C overnight. The next day, all sections were rinsed 3 times and incubated for 2 hours at room temperature in a mixture of Alexa Flour™ 555 donkey anti-mouse IgG (1:1000; Thermo Fisher; Cat. A31570) and Alexa Flour™ 488 donkey anti-rabbit IgG (1:1000; Thermo Fisher; Cat. A21206). After rinsing, the sections were incubated for 6 min in 4',6-diamidino-2-phenylindole (DAPI) (1:1000; Thermo Fisher; Cat. D21490) and coverslipped with antiuorescent quencher.
Thio avine-S staining Depara nized sections were incubated with 1% Thio avine-S (Sigma-Aldrich) for 5 min. Next, 70% ethanol was used to differentiate for 5 min, followed by rinsing with distilled water. The brain sections coverslipped with anti-uorescent quencher.
Thio avine-S staining Depara nized sections were incubated with 1% Thio avine-S (Sigma-Aldrich) for 5 min. Next, 70% ethanol was used to differentiate for 5 min, followed by rinsing with distilled water. The brain sections coverslipped with anti-uorescent quencher.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!