The sequences were analyzed using the Lasergene package version 10 (DNASTAR, Inc., USA) and deposited in the GenBank database under the following accession numbers: vaccine A, OM685193; vaccine B, OM685194; vaccine B_single passage, OM685195; vaccine C, OM685196. The nucleotide sequences were aligned by the Clustal W method using the DNASTAR 2.0 MegAlign program, and a phylogenetic tree was constructed by the neighbor-joining method with 1,000 bootstrap replicates using MEGA X software (version 10.2.2). For comparison, the nucleotide sequences of the S genes of the following strains were downloaded from the NCBI GenBank database: Conn48725 (FJ899692.1), Beaudette (M95169.1), M41 (AY561711.1), H120 (FJ888351.1), K046-12 (MK618758.1), BP-CaKII (MF924724.1), QXIBV (AF193423.1), K40/09 (HM486957.1), K047-12 (MK618759.1), ArkDPI (AF006624.1), GX2-98 (AY251816.1), K210-02 (AY257068.1), and Kr/D85/06 (EF621400.1). Chromatograms were reviewed manually using BioEdit version 7.2.5 to detect the presence of viral subpopulations in the vaccines.
2.0 megalign program
Manufactured by DNASTAR
The 2.0 MegAlign program is a sequence alignment software tool developed by DNASTAR. It is designed to align and compare DNA, RNA, or protein sequences to identify similarities and differences between them. The core function of the program is to perform multiple sequence alignment and phylogenetic analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 2.0 megalign program
1
Comparative Genomic Analysis of Avian Coronavirus Vaccines
2
Phylogenetic Analysis of SARS-CoV-2 Vaccine Variants
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The sequences received from Macrogen were analyzed using the Lasergene package version 10 (DNASTAR, Inc., USA) and deposited in GenBank as follows: Vaccine A, OM685193; Vaccine B, OM685194; Vaccine B_single passage, OM685195; Vaccine C, OM685196. The nucleotide sequences were aligned via the Clustal W method using the DNASTAR 2.0 MegAlign program and the phylogenetic tree was constructed via the neighbor-joining method with 1,000 bootstrap replicates using MEGA X software (version 10.2.2). The nucleotide sequences of the S glycoproteins, for comparison, were downloaded from the NCBI GenBank database as follows: Conn48725 (FJ899692.
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