Gemini nx 5 μ c18 110a column

IR spectra were obtained using JASCO FTIR 4100 spectrometer in CH₃OH solvent. Optical rotations were measured on a JASCO DIP-1000 polarimeter (Tokyo, Japan). HRESIMS data were measured on maXis 4G (Bruker) and LCQ Fleet (Thermoscientific), respectively. NMR spectra were recorded on a Bruker Avance 400, 500 and 800 MHz spectrometers using CD₃OD as solvent. Silica gel (200–400 mesh, Merck), Sephadex LH-20 (20–100 μm, Sigma) and Diaion HP-20P (Mitsubishi Kasei, Japan) for column chromatography. TLC was performed on silica gel 60 F₂₅₄ (0.2 mm, Merck) or silica gel 60 RP-18 F_254S (0.2 mm, Merck), and spots were detected by a 10% vanillin-H₂SO₄ in EtOH spray reagent. MPLC was performed on a Biotage Isolera Prime chromatography system and a Lichroprep RP-18 column (40–63 μm). Semi-prep HPLC was performed using a Waters system (three 515 pumps and a 996 photodiode array detector) with a Phenomenex Gemini-NX 5 μ C18 110A column (USA). Details of NMR and HPLC methodology as well as NMR/NOESY spectra of new compounds are present in Supplementary Information.