Hydrochloric acid (hcl)

Solketal (DL – 1,2 – Isopropylidineglycerol), ferulic acid, vinyl acetate and 11-bromoundecanoic acid were purchased from Sigma. Potassium hydroxide, mercuric acetate, sulphuric acid, hydrochloric acid, silica gel for column chromatography were purchased from SRL chemicals, merck, Avera, Advent and Acme Synthetic Chemicals, Mumbai, India respectively. All solvents were procured from Merck and were of highest grade of purity. Immobilized lipase from Candida antarctica (CAL‐B) was procured from Novozymes Bagsvaerd, Denmark).
The proton NMR spectra were recorded on Varian 500/300 and 125/75 MHz instrument and TMS was used as an internal standard. Mass spectra were recorded using electron spray ionization on Waters e2695 Seperators module (Waters, Milford, MA, USA) mass spectrometer. Infra red (IR) spectra were recorded using a Perkin‐Elmer Fourier transform (FT)‐IR Spectrum BX instrument (Model: Spectrum BX; Connecticut, USA). All the synthesized compounds were purified by silica gel (60–120 mesh) column chromatography (Acme Synthetic Chemicals, Mumbai, India) and were identified by thin‐layer chromatography (TLC). TLC was performed on precoated silica gel 60 F₂₅₄ from Merck (Darmstadt, Germany).

P. monodon (Fabricius, 1798) shells were collected from the Estuary of Southeast coast of Mandapam, Tamil Nadu, India. Sodium hydroxide, Acetone, Ethanol and Hydrochloric acid used were purchased from Sisco Research Laboratories Pvt. Ltd., Mumbai, India, and Dialysis membrane was purchased from HiMedia Laboratories, Mumbai, India. Methylene Blue, Bromophenol Blue and Coomassie Brilliant Blue were purchased from Sigma–Aldrich, USA.