Total RNA was extracted using RNA prep pure plant kit (Tiangen, Beijing, China), then 1.5 μg RNA was used to synthesize first-strand cDNA using M5 Super qPCR RT kit with gDNA remover (Mei5 biotechnology, Beijing, China), and then qRT-PCR analysis was conducted using the 2 × Real time PCR Super mix (SYBRgreen) (Mei5 biotechnology, Beijing, China) on the ABI 7500 thermocycler (Applied Biosystems). Ubi2 gene (UniProtKB/TrEMBL, Q42415) provided a control, and the 2−ΔΔCt method was used to calculate the expression.
M5 super qpcr rt kit with gdna remover
Manufactured by Mei5 Biotechnology
Sourced in China
The M5 Super qPCR RT kit with gDNA remover is a real-time quantitative PCR (qPCR) reagent kit that includes a genomic DNA (gDNA) removal step. The kit is designed for the reverse transcription and amplification of RNA targets.
Automatically generated - may contain errors
Lab products found in correlation
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Abi 7500 thermocycler, by Thermo Fisher Scientific (1 mentions)
Rnaprep pure plant kit, by Tiangen Biotech (1 mentions)
Smarter race 5 3 kit, by Takara Bio (1 mentions)
Rnaclean kit, by Aidlab (1 mentions)
Easyspin kit, by Aidlab (1 mentions)
Hiper sybr premix estaq, by Mei5 Biotechnology (1 mentions)
Cfx96 real time system, by Bio-Rad (1 mentions)
2 protocols using m5 super qpcr rt kit with gdna remover
1
Quantitative Real-Time PCR Assay
2
Comprehensive RNA Extraction and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total RNAs used for RACE and RNA‐seq were extracted using Trizol reagent (Thermo Fisher Scientific) and purified using the RNAclean Kit (Aidlab Biotechnologies). Total RNAs used for RT‐PCR were extracted using EASYspin Kit (Aidlab Biotechnologies), following manufacturer's instructions. First‐strand cDNA was synthesized using M5 Super qPCR RT Kit with gDNA remover (Mei5bio). The 5′‐ and 3′‐ RACE were conducted with SMARTer RACE 5′/3′ kit (TaKaRa) following manufacturer's instructions. Real‐time PCR was performed using a CFX96 real‐time system (Bio‐Rad) and Hiper SYBR Premix Es Taq (Mei5bio). Three replicates were performed and rice ACTIN1 was used as the internal control (Livak and Schmittgen, 2001 (link)). Primers used for qRT‐PCR are listed in Table S2 .
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