The monoclonal antibodies against mouse IL-2, CD8, and NK1.1 were obtained from hybridoma clones, S4B6-1, 53-6.72, and PK-136, respectively (American Type Culture Collection, USA). The recombinant mouse IL-2 (rIL-2) was purchased from BioLegend (USA). For the preparation of IL-2C, 1.5 μg of rIL-2 and 15 μg of anti-IL-2 were mixed in vitro, and then IL-2C was i.p. injected two times per every week at 3-day or 4-day intervals from 100 days of age until endpoint. For the isotype control, the same amount of purified rat IgG2a (BioLegend) was used. For depletion of CD8+-T cells or NK-T cells and NK cells, 100 μg of anti-CD8 or anti-NK1.1 was i.p. injected two times per every week at 3-day or 4-day intervals from 100 days of age until endpoint. Equal volume of PBS was used as the control. The efficacies of these administrations were confirmed by flow cytometry.
Pk 136
Manufactured by American Type Culture Collection
Sourced in United States
The PK-136 is a laboratory equipment used for the separation and purification of biological samples. It functions as a centrifuge, utilizing centrifugal force to separate components within a mixture based on their respective densities.
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Lab products found in correlation
3 protocols using pk 136
1
Modulating immune responses with IL-2C
2
NK/NKT Cell Depletion in GNMT-/- Mice
3
NK/NKT Cell Depletion in GNMT-/- Mice
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NK/NKT and specific NK cell depletion was achieved by i.p. administration of 250 µg/mouse of NK1.1+ antibody purified from hybridoma PK-136 (American Type Culture Collection) culture supernatants as previously described 19 (link) and by ASIALO respectively. Both ASIALO and NK1.1+ Abs were injected 24h before BDL and every 72h after surgery. As control group, GNMT−/− mice were injected with IgG (250 µg/mouse from BD bioscience) at the same timepoints as ASIALO and NK1.1+ Ab.
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