Pbd b peo

1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (16:0 PC or DPPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2–1,3-benzoxadiazol-4-yl) (16:0 NBD PE or NBD-DPPE) were purchased from Avanti Polar Lipids (Alabaster; AL, USA). Amphiphilic diblock copolymer, poly(butadiene-b-ethylene oxide) (PBD-b-PEO), was purchased from Polymer Source, Inc. (Quebec, Canada). The catalog number is P19015-BdEO. Its average molecular weight (Mn) was reported to be 4000 with PBD block (rich in 1,4 microstructure) 2500 and PEO block 1500, respectively. The reported polydispersity was 1.06. In this work, we selected PBD-b-PEO since the previous studies successfully demonstrated that the mixtures of phospholipids/PBD-b-PEO form phase-separated hybrid vesicles with domains.^{25 (link),35} For biomedical applications, the use of biocompatible polymer blocks such as polyisobutylene or polyoxazoline is advised. Paclitaxel, its fluorescent conjugate Paclitaxel-Oregon Green 488, and DilC18(5) oil (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate) were purchased from Thermo Fisher Scientific (Waltham; MA, USA). All solvents used were of high performance liquid chromatography (HPLC) grade and purchased from Sigma-Aldrich (St Louis; MO, USA). All chemicals and materials were used as received.

1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) suspended in chloroform were obtained from Avanti Polar Lipids (Alabama, USA). Amphiphilic diblock copolymer, poly(butadiene-b-ethylene oxide) (PBD-b-PEO), catalog number P19015-BdEO, was acquired from Polymer Source, Inc. (Quebec, Canada). The polydispersity is 1.06 and its average molecular weight (Mn) is 4000 with PBD block (rich in 1,4 microstructure) 2500 and PEO block 1500, respectively. In this study, we chose PBD-b-PEO since the previous work showed formation of phase-separated hybrid vesicles with domains^{38 (link)–40} . Paclitaxel was purchased from Thermo Fisher Scientific (Waltham; MA, USA). DSM265 was purchased from MedChemExpress (MedChemExpress LLC; NJ, USA). All chemicals and materials were used as received.