M199 media for promastigote culturing, Roswell Park Memorial Institute (RPMI) 1640 media for cell line, penicillin–streptomycin antibiotic cocktail, fetal bovine serum (FBS) were purchased from Gibco, Thermo Fisher Scientific. HEPES, sodium bicarbonate and paraformaldehyde were procured from Sigma–Aldrich. Miltefosine, 3-(4,5 dimethyl- thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay reagents and cell culture-grade dimethyl sulfoxide (DMSO) were purchased from Merck & Co., Inc. Propidium iodide, RNase A and reactive oxygen species (ROS) dye (H2DCFDA) were procured from Thermo Scientific. Cynaroside was purchased from ChemScene India. All the other chemicals and reagents were purchased from Sigma–Aldrich or Merck, unless stated otherwise.
3 4 5 dimethyl thiazol 2 yl 2 5 diphenyl tetrazolium bromide mtt assay reagents
Manufactured by Merck Group
3-(4,5 dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is a colorimetric assay reagent used to measure cell metabolic activity. It is a yellow tetrazolium salt that is reduced by metabolically active cells, producing a purple formazan product.
Automatically generated - may contain errors
Lab products found in correlation
Propidium iodide, by Thermo Fisher Scientific (6 mentions)
Cell culture grade dimethyl sulfoxide dmso, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin antibiotic cocktail, by Thermo Fisher Scientific (1 mentions)
Hepes, by Merck Group (1 mentions)
Rnase a, by Thermo Fisher Scientific (1 mentions)
Sodium bicarbonate, by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Miltefosine, by Merck Group (1 mentions)
2 protocols using 3 4 5 dimethyl thiazol 2 yl 2 5 diphenyl tetrazolium bromide mtt assay reagents
1
Leishmania promastigote culture and cytotoxicity
2
Promastigote and Cell Line Culturing
Check if the same lab product or an alternative is used in the 5 most similar protocols
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M199 media
for promastigote culturing, Roswell Park Memorial Institute (RPMI)
1640 media for the cell line, penicillin–streptomycin antibiotic
cocktail, and fetal bovine serum (FBS) were procured from Gibco and
Thermo Fisher Scientific, Inc., Waltham, MA. N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES), sodium bicarbonate,
paraformaldehyde were obtained from Sigma-Aldrich, Saint Louis, MO.
Miltefosine, 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium
bromide (MTT) assay reagents, and cell culture-grade dimethyl sulfoxide
(DMSO) and other solvents for plant extract were from Merck &
Co., Inc., Kenilworth, NJ. Propidium iodide and Annexin fluorescein
isothiocyante (FITC) was procured from Thermo Fisher Scientific, Inc.,
Waltham, MA. All of the other chemicals and reagents were purchased
from Sigma-Aldrich, Saint Louis, MO, or Merck & Co., Inc., Kenilworth,
NJ, unless stated otherwise.
for promastigote culturing, Roswell Park Memorial Institute (RPMI)
1640 media for the cell line, penicillin–streptomycin antibiotic
cocktail, and fetal bovine serum (FBS) were procured from Gibco and
Thermo Fisher Scientific, Inc., Waltham, MA. N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES), sodium bicarbonate,
paraformaldehyde were obtained from Sigma-Aldrich, Saint Louis, MO.
Miltefosine, 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium
bromide (MTT) assay reagents, and cell culture-grade dimethyl sulfoxide
(DMSO) and other solvents for plant extract were from Merck &
Co., Inc., Kenilworth, NJ. Propidium iodide and Annexin fluorescein
isothiocyante (FITC) was procured from Thermo Fisher Scientific, Inc.,
Waltham, MA. All of the other chemicals and reagents were purchased
from Sigma-Aldrich, Saint Louis, MO, or Merck & Co., Inc., Kenilworth,
NJ, unless stated otherwise.
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