Spr 220

Manufactured by MicroChem

The SPR-220 is a lab equipment product that serves as a surface plasmon resonance (SPR) instrument. Its core function is to detect and measure interactions between molecules in real-time, without the need for labeling. The SPR-220 is designed to provide accurate and reliable data for various applications in the fields of biochemistry, biophysics, and drug discovery.

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Lab products found in correlation

Fibronectin, by Merck Group (1 mentions) Bind silane, by Merck Group (1 mentions) Alexa fluor 568 dye, by Thermo Fisher Scientific (1 mentions) Basement membrane extract, by Bio-Techne (1 mentions) 2 meoe2, by Selleck Chemicals (1 mentions) Linifanib, by Selleck Chemicals (1 mentions) Ham s f12 medium, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Su 8 developer, by MicroChem (1 mentions) Su 8 2015 photoresist, by MicroChem (1 mentions)

Spelling variants of this product

SPR 220.3 positive photoresist (2 citations) SPR 220-7 (2 citations)

2 protocols using spr 220

Linear Micropattern Fabrication and Functionalization

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Linear micropatterned strips were prepared by blocking areas outside the strip with linear polyacrylamide as described previously (Guo and Wang, 2011 (link)). Briefly, coverslips were activated with Bind-Silane (Sigma-Aldrich), and areas for cell adhesion were covered with a positive photoresist SPR-220 (MicroChem, Newton, MA). Following development of the micropattern, the uncovered areas outside the strip were made nonadhesive by grafting linear polyacrylamide to the Bind-Silane-activated glass surface. Photoresist-protected adhesive regions were then exposed by stripping off the photoresist. The micropatterned substrates, as well as unpatterned substrates, were incubated for 30 min with 20 µg/ml fibronectin (Sigma-Aldrich) with or without the conjugation of Alexa Fluor 568 dye (Invitrogen, Eugene, OR), before plating the cells.

Zhang J, & Wang Y.L. (2017). Centrosome defines the rear of cells during mesenchymal migration. Molecular Biology of the Cell, 28(23), 3240-3251.

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M-Chip Microfluidic Device Fabrication

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Silicon wafers (4-inch) were purchased from Corning Inc. (Corning, NY). SPR-220, SU-8 2015 photoresist, and SU-8 developer were purchased from MicroChem Corp. (Newton, MA). MF-CD26 was obtained from Rohm and Haas Electronic Materials (Marlborough, MA). Polydimethylsiloxane (PDMS RTV615) was obtained from Momentive Performance Materials (Waterford, NY). Ham’s F12 medium and fetal bovine serum (FBS) was obtained from Invitrogen (Grand Island, NY). Basement Membrane Extract (BME) was obtained from Trevigen (Gaithersburg, MD). Phosphate-buffered saline (PBS; 0.1 M, pH 7.4) was obtained from Lonza Inc. (Allendale, NJ). All M-Chip devices were designed as computer graphics using AutoCAD software and then printed out as 10-μm–resolution chrome masks by Photo Science Inc. (Torrance, CA). The SUM-159 cell line was obtained from Asterand (Detroit, MI). Linifanib and 2-MeOE2 were obtained from Selleckchem (Houston, TX). Drug 227013 was obtained from EMD Millipore (Darmstadt, Germany).

Zhang Y., Wen J., Zhou L, & Qin L. (2015). Utilizing a High-Throughput Microfluidic Platform to Study Hypoxia-Driven Mesenchymal-Mode Cell Migration. Integrative biology : quantitative biosciences from nano to macro, 7(6), 672-680.

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