The enzymatic degradation of GEL_bulk and GEL_porous was evaluated by in vitro tests using collagenase Type I from Clostridium Histolyticum (Worthington Biomedical) [43] (link). Specimens (n = 3 per type per time point, Ø = 6 mm) were disinfected by immersion overnight in 70% v/v solution, allowed to dry and weighted (w0) in sterile condition. Then, samples were placed in sterile Eppendorf tubes, immersed in 500 µL collagenase solution 1 U/mL in PBS [43] (link) or in PBS, as control, and stored in an orbital shaker incubator (37 °C, 100 rpm). At fixed time points (i.e., 1, 2, 3, 4, 6, 24 and 48 h) samples were washed three times with PBS, frozen, lyophilized and weighted (wt). The residual weight (RW%) was calculated as ratio of the weight at the time point t to the initial weight, according to formula (1), and represented in time to investigate the gelatin hydrogels degradation.
Collagenase type 1 from clostridium histolyticum
Manufactured by Worthington
Sourced in Japan
Collagenase Type I from Clostridium Histolyticum is an enzyme used for the dissociation of tissues. It acts by cleaving the peptide bonds in collagen, a major structural component of the extracellular matrix.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle s medium nutrient mixture f 12 ham dmem f 12, by Merck Group (1 mentions)
Human serum, by Merck Group (1 mentions)
Trypsin edta solution, by Merck Group (1 mentions)
Penicillin streptomycin solution, by Merck Group (1 mentions)
Dab peroxidase substrate kit, by Vector Laboratories (1 mentions)
Vectastain elite abc kit, by Vector Laboratories (1 mentions)
Biotinylated secondary antibody, by Vector Laboratories (1 mentions)
2 protocols using collagenase type 1 from clostridium histolyticum
1
Enzymatic Degradation of Gelatin Hydrogels
2
Engineered Cartilage Regeneration
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Dulbecco's Modified Eagle's Medium/Nutrient Mixture F-12 Ham (DMEM/F12), human serum, penicillin-streptomycin solution, and trypsin–EDTA solution were purchased from Sigma Chemical Co. (MO, USA), IGF-I was from Astellas Pharma Inc (Tokyo, Japan), FGF-2 was from Kaken Pharmaceutical Co, Ltd. (Tokyo, Japan), and insulin was from Novo Nordisk Pharma Ltd. (Tokyo, Japan). One percent atelocollagen was purchased from Kawaken Fine Chemicals Co., Ltd. (Tokyo, Japan), 3% atelocollagen from Koken Co., Ltd. (Tokyo, Japan), poly-L lactic acid (PLLA) scaffolds from GC R&D Dept. (Tokyo, Japan), collagenase, type I from Clostridium histolyticum from Worthington Biochemical Corporation, collagenase from C. histolyticum and anti-collagen antibody (type I and II) from LSL CO., LTD, TdT-mediated dUTP nick end labeling kit (TUNEL) from TAKARA Bio Inc. (Tokyo, Japan), anti-proliferating cell nuclear antigen antibody (PCNA) from Abcam® plc (Tokyo, Japan). Biotinylated secondary antibody, Vectastain Elite ABC Kit, and DAB Peroxidase Substrate Kit were all obtained from Vector Laboratories (Burlingame, CA).
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