K pneumoniae atcc 2146

All laboratory strains, including Escherichia coli ATCC 25922, K pneumoniae ATCC 2146, and P aeruginosa ATCC 27853, were obtained from the American Type Culture Collection (ATCC). A total of 60 clinical bacterial strains were tested, including 30 K pneumoniae and 30 P aeruginosa. All clinical isolates were recovered from two tertiary care hospitals in Cali, Colombia. The isolates were from intensive care units, and the sample sources were respiratory secretions, urine, and blood. The cultures of isolates were sent to a Microbiology Laboratory at Laboratorio de Salud Publica Departamental del Valle del Cauca (LSPD-Valle), in the context of laboratory surveillance, where the bacterial species were confirmed, and the antibiotic susceptibility characterization was performed. The strains were identified with the VITEK 2 Gram-Negative identification (VITEK 2 GN ID) card based on established substrates and biochemical methods measuring carbon source utilization, enzymatic activities, and resistance (Ref. 21341, Biomérieux). The VITEK 2 GN ID card was used with the VITEK 2 system for the automated identification of Enterobacteriaceae and non-Enterobacteriaceae Gram-negative bacilli.