Hotair

The dulbecco’s modified Eagle’s medium (DMEM) was obtained from Gibco; Thermo Fisher Scientific, Inc. (Waltham, MA, USA). The 10% heat-inactivated fetal bovine serum (FBS) was achieved from Hyclone (Logan, USA) while the 1% antibiotic/antimycotic solution was from Sigma-Aldrich, Merck KGaA (Darmstadt, Germany). The lentivirus respectively containing HOTAIR, miR-488-5p, si-HOTAIR, si-miR-488-5p and negative control (NC) were purchased from Shanghai Gene Pharma Company (Shanghai, China). 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The primary antibodies and horseradish peroxidase conjugated IgG were obtained from BD Bioscience (San Diego, CA, USA). The reagents used here were all analytical grade and obtained from Aladdin reagent co., Ltd. (Shanghai, China) if not mention elsewhere.